9NS5

Get3(D57N)-Get4/5 Complex (ATP-bound)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (C, F)	Ubiquitin-like protein MDY2	polymer	212	23768.3	2	UniProt (Q12285) Pfam (PF16843) Pfam (PF00240) Pfam (PF18514)	Saccharomyces cerevisiae S288C	Golgi to ER traffic protein 5,Mating-deficient protein 2,Translation machinery-associated protein 24
2	C, E (A, D)	ATPase GET3	polymer	376	41936.3	2	UniProt (Q12154) Pfam (PF02374)	Saccharomyces cerevisiae S288C	Arsenical pump-driving ATPase,Arsenite-stimulated ATPase,Golgi to ER traffic protein 3,Guided entry of tail-anchored proteins 3
3	D, F (B, E)	Golgi to ER traffic protein 4	polymer	334	38604.4	2	UniProt (Q12125) Pfam (PF04190)	Saccharomyces cerevisiae S288C	Guided entry of tail-anchored proteins 4
4	G (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	H, J (A, D)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
6	I, K (A, D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, D: 1 - 354 (UniProt: Q12154)

PDB	External Database	Details
Met -21	-	initiating methionine
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Asn 57	Asp 57	engineered mutation

B, E: 1 - 312 (UniProt: Q12125)

PDB	External Database	Details
Ser 313	-	expression tag
Gly 314	-	expression tag
Gly 315	-	expression tag
Gly 316	-	expression tag
Gly 317	-	expression tag
Gly 318	-	expression tag
Glu 319	-	expression tag
Asn 320	-	expression tag
Leu 321	-	expression tag
Tyr 322	-	expression tag
Phe 323	-	expression tag
Gln 324	-	expression tag
Gly 325	-	expression tag
Gly 326	-	expression tag
Ser 327	-	expression tag
Ser 328	-	expression tag
His 329	-	expression tag
His 330	-	expression tag
His 331	-	expression tag
His 332	-	expression tag
His 333	-	expression tag
His 334	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	208618.2
Non-Polymers*	Number of molecules	5
	Total formula weight	1128.4
All*	Total formula weight	209746.6

*Water molecules are not included.