9NQU

KDM6B-nucleosome structure stabilized by H3K27C-UNC8015 covalent conjugate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.2	polymer	135	15231.8	2	UniProt (Q71DI3) Pfam (PF00125)	Homo sapiens (human)	H3-clustered histone 13,H3-clustered histone 14,H3-clustered histone 15,Histone H3/m,Histone H3/o
2	B, F (B, F)	Histone H4	polymer	102	11263.2	2	UniProt (P62805) Pfam (PF15511)	Homo sapiens (human)
3	C, G (C, G)	Histone H2A type 1	polymer	129	13990.3	2	UniProt (P0C0S8) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (human)	H2A.1,Histone H2A/ptl
4	D, H (D, H)	Histone H2B type 1-C/E/F/G/I	polymer	125	13806.0	2	UniProt (P62807) Pfam (PF00125)	Homo sapiens (human)	Histone H2B.1 A,Histone H2B.a,H2B/a,Histone H2B.g,H2B/g,Histone H2B.h,H2B/h,Histone H2B.k,H2B/k,Histone H2B.l,H2B/l
5	I (I)	DNA (185-MER)	polymer	185	56831.2	1		synthetic construct
6	J (J)	DNA (185-MER)	polymer	185	57400.6	1		synthetic construct
7	K (K)	Lysine-specific demethylase 6B	polymer	514	58169.4	1	UniProt (Q5NCY0) Pfam (PF02373) Pfam (PF21322) Pfam (PF21326)	Mus musculus (house mouse)	JmjC domain-containing protein 3,Jumonji domain-containing protein 3,[histone H3]-trimethyl-L-lysine(27) demethylase 6B
8	L (K)	FE (III) ION	non-polymer		55.8	1	Chemie (FE)
9	M (K)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
10	N (K)	N-heptanoyl-N-hydroxy-beta-alanine	non-polymer		217.3	1	Chemie (OH0)

Sequence modifications

A, E: 1 - 135 (UniProt: Q71DI3)

PDB	External Database	Details
Cys 27	Lys 28	conflict
Ala 110	Cys 111	conflict

K: 1130 - 1641 (UniProt: Q5NCY0)

PDB	External Database	Details
Gly 1128	-	expression tag
Ser 1129	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	280983.9
Non-Polymers*	Number of molecules	3
	Total formula weight	338.5
All*	Total formula weight	281322.4

*Water molecules are not included.