9NO7
Cryo-EM structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys at 2.13A resolution
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | 23S Ribosomal RNA | polymer | 2915 | 948007.6 | 1 | GenBank (55771382) | Thermus thermophilus HB8 | |
| 2 | B (B) | 5S Ribosomal RNA | polymer | 121 | 39188.4 | 1 | GenBank (AP008226.1) | Thermus thermophilus HB8 | |
| 3 | C (D) | 50S ribosomal protein L2 | polymer | 276 | 30532.6 | 1 | UniProt (P60405) Pfam (PF00181) Pfam (PF03947) | Thermus thermophilus HB8 | |
| 4 | D (E) | 50S ribosomal protein L3 | polymer | 206 | 22450.2 | 1 | UniProt (Q5SHN8) Pfam (PF00297) | Thermus thermophilus HB8 | |
| 5 | E (F) | 50S ribosomal protein L4 | polymer | 210 | 23271.9 | 1 | UniProt (Q5SHN9) Pfam (PF00573) | Thermus thermophilus HB8 | L1e |
| 6 | F (G) | 50S ribosomal protein L5 | polymer | 182 | 21061.6 | 1 | UniProt (Q5SHQ0) Pfam (PF00281) Pfam (PF00673) | Thermus thermophilus HB8 | |
| 7 | G (H) | 50S ribosomal protein L6 | polymer | 180 | 19568.9 | 1 | UniProt (P0DOY8) Pfam (PF00347) | Thermus thermophilus HB8 | |
| 8 | H (I) | 50S ribosomal protein L9 | polymer | 148 | 16422.2 | 1 | UniProt (Q5SLQ1) Pfam (PF01281) Pfam (PF03948) | Thermus thermophilus HB8 | |
| 9 | I (N) | 50S ribosomal protein L13 | polymer | 140 | 15927.9 | 1 | UniProt (P60488) Pfam (PF00572) | Thermus thermophilus HB8 | |
| 10 | J (O) | 50S ribosomal protein L14 | polymer | 122 | 13323.6 | 1 | UniProt (Q5SHP8) Pfam (PF00238) | Thermus thermophilus HB8 | |
| 11 | K (P) | 50S ribosomal protein L15 | polymer | 150 | 16319.1 | 1 | UniProt (Q5SHQ7) Pfam (PF00828) | Thermus thermophilus HB8 | |
| 12 | L (Q) | 50S ribosomal protein L16 | polymer | 141 | 15993.9 | 1 | UniProt (P60489) Pfam (PF00252) | Thermus thermophilus HB8 | |
| 13 | M (R) | 50S ribosomal protein L17 | polymer | 118 | 13750.1 | 1 | UniProt (Q9Z9H5) Pfam (PF01196) | Thermus thermophilus HB8 | |
| 14 | N (S) | 50S ribosomal protein L18 | polymer | 112 | 12639.8 | 1 | UniProt (Q5SHQ4) Pfam (PF00861) | Thermus thermophilus HB8 | TL24 |
| 15 | O (T) | 50S ribosomal protein L19 | polymer | 146 | 17188.8 | 1 | UniProt (P60490) Pfam (PF01245) | Thermus thermophilus HB8 | |
| 16 | P (U) | 50S ribosomal protein L20 | polymer | 118 | 13779.3 | 1 | UniProt (P60491) Pfam (PF00453) | Thermus thermophilus HB8 | |
| 17 | Q (V) | 50S ribosomal protein L21 | polymer | 101 | 11069.2 | 1 | UniProt (P60492) Pfam (PF00829) | Thermus thermophilus HB8 | |
| 18 | R (W) | 50S ribosomal protein L22 | polymer | 113 | 12808.1 | 1 | UniProt (Q5SHP3) Pfam (PF00237) | Thermus thermophilus HB8 | |
| 19 | S (X) | 50S ribosomal protein L23 | polymer | 96 | 10759.8 | 1 | UniProt (Q5SHP0) Pfam (PF00276) | Thermus thermophilus HB8 | |
| 20 | T (Y) | 50S ribosomal protein L24 | polymer | 110 | 12085.6 | 1 | UniProt (Q5SHP9) Pfam (PF00467) Pfam (PF17136) | Thermus thermophilus HB8 | |
| 21 | U (Z) | 50S ribosomal protein L25 | polymer | 206 | 23238.6 | 1 | UniProt (Q5SHZ1) Pfam (PF01386) Pfam (PF14693) | Thermus thermophilus HB8 | TL5 |
| 22 | V (0) | 50S ribosomal protein L27 | polymer | 85 | 9529.1 | 1 | UniProt (P60493) Pfam (PF01016) | Thermus thermophilus HB8 | |
| 23 | W (1) | 50S ribosomal protein L28 | polymer | 98 | 11004.2 | 1 | UniProt (P60494) Pfam (PF00830) | Thermus thermophilus HB8 | |
| 24 | X (2) | 50S ribosomal protein L29 | polymer | 72 | 8670.3 | 1 | UniProt (Q5SHP6) Pfam (PF00831) | Thermus thermophilus HB8 | |
| 25 | Y (3) | 50S ribosomal protein L30 | polymer | 60 | 6799.1 | 1 | UniProt (Q5SHQ6) Pfam (PF00327) | Thermus thermophilus HB8 | |
| 26 | Z (4) | 50S ribosomal protein L31 | polymer | 71 | 8300.5 | 1 | UniProt (Q5SJE1) Pfam (PF01197) | Thermus thermophilus HB8 | |
| 27 | AA (5) | 50S ribosomal protein L32 | polymer | 60 | 6722.1 | 1 | UniProt (P80339) Pfam (PF01783) | Thermus thermophilus HB8 | |
| 28 | BA (6) | 50S ribosomal protein L33 | polymer | 54 | 6632.9 | 1 | UniProt (P35871) Pfam (PF00471) | Thermus thermophilus HB8 | |
| 29 | CA (7) | 50S ribosomal protein L34 | polymer | 49 | 6132.4 | 1 | UniProt (P80340) Pfam (PF00468) | Thermus thermophilus HB8 | |
| 30 | DA (8) | 50S ribosomal protein L35 | polymer | 65 | 7506.2 | 1 | UniProt (Q5SKU1) Pfam (PF01632) | Thermus thermophilus HB8 | |
| 31 | EA (9) | 50S ribosomal protein L36 | polymer | 37 | 4435.4 | 1 | UniProt (Q5SHR2) Pfam (PF00444) | Thermus thermophilus HB8 | Ribosomal protein B |
| 32 | FA (a) | 16S Ribosomal RNA | polymer | 1521 | 493863.6 | 1 | GenBank (55771382) | Thermus thermophilus HB8 | |
| 33 | GA (b) | 30S ribosomal protein S2 | polymer | 256 | 29317.7 | 1 | UniProt (P80371) Pfam (PF00318) | Thermus thermophilus HB8 | |
| 34 | HA (c) | 30S ribosomal protein S3 | polymer | 239 | 26751.1 | 1 | UniProt (P80372) Pfam (PF07650) Pfam (PF00189) | Thermus thermophilus HB8 | |
| 35 | IA (d) | 30S ribosomal protein S4 | polymer | 209 | 24373.4 | 1 | UniProt (P80373) Pfam (PF00163) Pfam (PF01479) | Thermus thermophilus HB8 | |
| 36 | JA (e) | 30S ribosomal protein S5 | polymer | 162 | 17583.4 | 1 | UniProt (Q5SHQ5) Pfam (PF00333) Pfam (PF03719) | Thermus thermophilus HB8 | |
| 37 | KA (f) | 30S ribosomal protein S6 | polymer | 101 | 11988.8 | 1 | UniProt (Q5SLP8) Pfam (PF01250) | Thermus thermophilus HB8 | TS9 |
| 38 | LA (g) | 30S ribosomal protein S7 | polymer | 156 | 18051.0 | 1 | UniProt (P17291) Pfam (PF00177) | Thermus thermophilus HB8 | |
| 39 | MA (h) | 30S ribosomal protein S8 | polymer | 138 | 15868.6 | 1 | UniProt (P0DOY9) Pfam (PF00410) | Thermus thermophilus HB8 | |
| 40 | NA (i) | 30S ribosomal protein S9 | polymer | 128 | 14410.6 | 1 | UniProt (P80374) Pfam (PF00380) | Thermus thermophilus HB8 | |
| 41 | OA (j) | 30S ribosomal protein S10 | polymer | 105 | 11955.0 | 1 | UniProt (Q5SHN7) Pfam (PF00338) | Thermus thermophilus HB8 | |
| 42 | PA (k) | 30S ribosomal protein S11 | polymer | 129 | 13737.9 | 1 | UniProt (P80376) Pfam (PF00411) | Thermus thermophilus HB8 | |
| 43 | QA (l) | 30S ribosomal protein S12 | polymer | 132 | 14683.5 | 1 | UniProt (Q5SHN3) Pfam (PF00164) | Thermus thermophilus HB8 | |
| 44 | RA (m) | 30S ribosomal protein S13 | polymer | 126 | 14338.9 | 1 | UniProt (P80377) Pfam (PF00416) | Thermus thermophilus HB8 | |
| 45 | SA (n) | 30S ribosomal protein S14 type Z | polymer | 61 | 7158.7 | 1 | UniProt (P0DOY6) Pfam (PF00253) | Thermus thermophilus HB8 | |
| 46 | TA (o) | 30S ribosomal protein S15 | polymer | 89 | 10578.4 | 1 | UniProt (Q5SJ76) Pfam (PF00312) | Thermus thermophilus HB8 | |
| 47 | UA (p) | 30S ribosomal protein S16 | polymer | 88 | 10410.0 | 1 | UniProt (Q5SJH3) Pfam (PF00886) | Thermus thermophilus HB8 | |
| 48 | VA (q) | 30S ribosomal protein S17 | polymer | 105 | 12325.7 | 1 | UniProt (P0DOY7) Pfam (PF00366) | Thermus thermophilus HB8 | |
| 49 | WA (r) | 30S ribosomal protein S18 | polymer | 88 | 10258.3 | 1 | UniProt (Q5SLQ0) Pfam (PF01084) | Thermus thermophilus HB8 | |
| 50 | XA (s) | 30S ribosomal protein S19 | polymer | 93 | 10605.5 | 1 | UniProt (Q5SHP2) Pfam (PF00203) | Thermus thermophilus HB8 | |
| 51 | YA (t) | 30S ribosomal protein S20 | polymer | 106 | 11736.1 | 1 | UniProt (P80380) Pfam (PF01649) | Thermus thermophilus HB8 | |
| 52 | ZA (u) | 30S ribosomal protein Thx | polymer | 27 | 3350.0 | 1 | UniProt (Q5SIH3) Pfam (PF17070) | Thermus thermophilus HB8 | S31 |
| 53 | AB (v) | CYS-Stop mRNA | polymer | 24 | 7827.8 | 1 | Thermus thermophilus HB8 | ||
| 54 | BB (w) | Peptide chain release factor 1 | polymer | 354 | 40167.6 | 1 | UniProt (P96077) Pfam (PF03462) Pfam (PF00472) | Thermus thermophilus HB8 | RF-1 |
| 55 | CB (x) | P-site Peptidyl-tRNA fMEAAAKC-tRNAcys RNA-part | polymer | 74 | 23927.5 | 1 | Thermus thermophilus HB8 | ||
| 56 | DB (z) | P-site Peptidyl-tRNA fMEAAAKC-tRNAcys Peptide-part | polymer | 7 | 751.9 | 1 | Thermus thermophilus HB8 | ||
| 57 | AAA, AAB, ABA, ABB, AC... (A, B, D, E, O...) | MAGNESIUM ION | non-polymer | 24.3 | 1685 | Chemie (MG) | |||
| 58 | ADA, BCA, BDA, CCA, CDA... (A, Q) | POTASSIUM ION | non-polymer | 39.1 | 52 | Chemie (K) | |||
| 59 | KGB, KHB, OPB, WGB, XFB... (4, 9, n, 5, Y...) | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) | |||
| 60 | IPB (d) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 1 | Chemie (SF4) | |||
| 61 | ARB, BRB, CRB, DRB, ERB... (1, 2, 3, 5, 6...) | water | water | 18.0 | 4759 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 56 |
| Total formula weight | 2241140.1 | |
| Non-Polymers* | Number of molecules | 1744 |
| Total formula weight | 43731.1 | |
| All* | Total formula weight | 2284871.2 |
