9NNH
Cryo-EM structure of the Retron Ec78 complex, PtuA:PtuB:RT (4:2:2)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (C, D, E, F) | Retron I-A effector PtuA | polymer | 550 | 62467.8 | 4 | UniProt (P0DV91) | Escherichia coli | |
| 2 | E, H (J, K) | Retron I-A effector PtuB | polymer | 232 | 26879.2 | 2 | UniProt (P0DV92) | Escherichia coli | TIGR02646 family protein |
| 3 | F, G (B, A) | Retron I-A Ec78 reverse transcriptase | polymer | 311 | 35538.2 | 2 | UniProt (A0AB38GW94) | Escherichia coli | |
| 4 | I, L (N, M) | DNA (73-MER) | polymer | 78 | 24120.4 | 2 | Escherichia coli | ||
| 5 | J, K (L, X) | RNA (41-MER) | polymer | 67 | 21425.6 | 2 | Escherichia coli | ||
| 6 | M, N, O (C, D, F) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 3 | Chemie (ADP) |
Sequence modifications
C, D, E, F: 1 - 550 (UniProt: P0DV91)
J, K: 1 - 216 (UniProt: P0DV92)
B, A: 1 - 311 (UniProt: A0AB38GW94)
| PDB | External Database | Details |
|---|---|---|
| Ser 25 | Thr 25 | conflict |
| Lys 27 | Asn 27 | conflict |
| Ala 28 | Met 28 | conflict |
| Gly 37 | Asp 37 | conflict |
| Met 42 | Thr 42 | conflict |
| Ile 57 | Thr 57 | conflict |
| Leu 84 | Phe 84 | conflict |
| Met 130 | Leu 130 | conflict |
| Asp 139 | Asn 139 | conflict |
| Ser 142 | Ala 142 | conflict |
| Lys 180 | Arg 180 | conflict |
| Val 192 | Ala 192 | conflict |
| Asn 193 | Lys 193 | conflict |
| Ile 215 | Thr 215 | conflict |
| Thr 219 | Ala 219 | conflict |
| Thr 232 | Ala 232 | conflict |
| Val 254 | Ile 254 | conflict |
| Thr 274 | Ser 274 | conflict |
| Phe 291 | Leu 291 | conflict |
| Ile 297 | Ser 297 | conflict |
| Arg 307 | Lys 307 | conflict |
| Val 308 | Met 308 | conflict |
| Leu 309 | Val 309 | conflict |
| Ala 321 | Ser 321 | conflict |
| Asn 331 | Lys 331 | conflict |
| Ile 337 | Thr 337 | conflict |
| Ala 416 | Val 416 | conflict |
| Glu 444 | Asp 444 | conflict |
| Asp 450 | Asn 450 | conflict |
| Ser 478 | Pro 478 | conflict |
| Asn 491 | Asp 491 | conflict |
| His 506 | Gln 506 | conflict |
| Val 509 | Glu 509 | conflict |
| Gln 513 | Lys 513 | conflict |
| Ala 516 | Thr 516 | conflict |
| Ile 523 | Ala 523 | conflict |
| Leu 541 | Ile 541 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Arg 2 | Lys 2 | conflict |
| Arg 9 | Ser 9 | conflict |
| Ile 17 | Thr 17 | conflict |
| Gly 41 | Glu 41 | conflict |
| Cys 53 | Arg 53 | conflict |
| Asn 72 | Ser 72 | conflict |
| Arg 82 | Lys 82 | conflict |
| Phe 156 | Leu 156 | conflict |
| Thr 175 | Ser 175 | conflict |
| Asp 181 | Glu 181 | conflict |
| Tyr 201 | Phe 201 | conflict |
| Phe 211 | Ser 211 | conflict |
| Leu 217 | - | expression tag |
| Glu 218 | - | expression tag |
| Val 219 | - | expression tag |
| Leu 220 | - | expression tag |
| Phe 221 | - | expression tag |
| Gln 222 | - | expression tag |
| Gly 223 | - | expression tag |
| Pro 224 | - | expression tag |
| Glu 225 | - | expression tag |
| Ala 226 | - | expression tag |
| His 227 | - | expression tag |
| His 228 | - | expression tag |
| His 229 | - | expression tag |
| His 230 | - | expression tag |
| His 231 | - | expression tag |
| His 232 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 6 | Arg 6 | conflict |
| Asp 17 | Gly 17 | conflict |
| Ser 63 | Asn 63 | conflict |
| Leu 64 | Phe 64 | conflict |
| Gln 119 | Leu 119 | conflict |
| Leu 129 | Phe 129 | conflict |
| Arg 142 | His 142 | conflict |
| Lys 176 | Asn 176 | conflict |
| Val 178 | Leu 178 | conflict |
| Ser 193 | Cys 193 | conflict |
| Asp 197 | Asn 197 | conflict |
| Leu 206 | Ile 206 | conflict |
| Val 208 | Ala 208 | conflict |
| Ala 217 | Glu 217 | conflict |
| Ile 239 | Val 239 | conflict |
| Ile 278 | Thr 278 | conflict |
| Ser 290 | Gly 290 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 465797.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 1281.6 | |
| All* | Total formula weight | 467079.5 |
