9N9Q
Cryo-EM structure of the RNA-guided transcription complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Nuclease-deactivated Cas12f | polymer | 365 | 42913.3 | 2 | Flagellimonas taeanensis | ||
| 2 | C (C) | RpoE | polymer | 261 | 31011.5 | 1 | Flagellimonas taeanensis | ||
| 3 | D, E (D, E) | DNA-directed RNA polymerase subunit alpha | polymer | 330 | 37228.6 | 2 | UniProt (A0A1M6YWR9) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Flagellimonas taeanensis | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 4 | F (F) | DNA-directed RNA polymerase subunit beta | polymer | 1269 | 142961.4 | 1 | UniProt (A0A3A1NXR2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Flagellimonas taeanensis | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 5 | G (G) | DNA-directed RNA polymerase subunit beta' | polymer | 1432 | 160063.4 | 1 | UniProt (A0A3A1NXQ1) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Flagellimonas taeanensis | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 6 | H (H) | RNA polymerase Rpb6 | polymer | 108 | 12565.0 | 1 | UniProt (A0A1M7BX03) Pfam (PF01192) | Flagellimonas taeanensis | |
| 7 | I (a) | DNA non-template strand | polymer | 99 | 30316.4 | 1 | Flagellimonas taeanensis | ||
| 8 | J (b) | DNA template strand | polymer | 99 | 30761.7 | 1 | Flagellimonas taeanensis | ||
| 9 | K (c) | gRNA (85-MER) | polymer | 93 | 29922.8 | 1 | Flagellimonas taeanensis | ||
| 10 | L (G) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 11 | M (G) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
D, E: 14 - 330 (UniProt: A0A1M6YWR9)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Ala 2 | - | expression tag |
| Leu 3 | - | expression tag |
| Leu 4 | - | expression tag |
| Asn 5 | - | expression tag |
| Phe 6 | - | expression tag |
| Gln 7 | - | expression tag |
| Lys 8 | - | expression tag |
| Pro 9 | - | expression tag |
| Asp 10 | - | expression tag |
| Lys 11 | - | expression tag |
| Val 12 | - | expression tag |
| Ile 13 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 597885.8 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 89.7 | |
| All* | Total formula weight | 597975.5 |
