9N9P

Cryo-EM structure of the Fta RNAP complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	330	37228.6	2	UniProt (A0A1M6YWR9) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118)	Flagellimonas taeanensis	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (C)	DNA-directed RNA polymerase subunit beta	polymer	1269	142961.4	1	UniProt (A0A3A1NXR2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562)	Flagellimonas taeanensis	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D (D)	DNA-directed RNA polymerase subunit beta'	polymer	1432	160063.4	1	UniProt (A0A3A1NXQ1) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Flagellimonas taeanensis	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E (E)	RNA polymerase Rpb6	polymer	108	12565.0	1	UniProt (A0A1M7BX03) Pfam (PF01192)	Flagellimonas taeanensis
5	F (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	G (D)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A, B: 14 - 330 (UniProt: A0A1M6YWR9)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag
Leu 3	-	expression tag
Leu 4	-	expression tag
Asn 5	-	expression tag
Phe 6	-	expression tag
Gln 7	-	expression tag
Lys 8	-	expression tag
Pro 9	-	expression tag
Asp 10	-	expression tag
Lys 11	-	expression tag
Val 12	-	expression tag
Ile 13	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	390046.9
Non-Polymers*	Number of molecules	2
	Total formula weight	89.7
All*	Total formula weight	390136.6

*Water molecules are not included.