9N8L

Glutarate L-2-hydroxylase N182A, Q184C, K270A mutant-5'-Mal-C2-AGCT DNA conjugate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Glutarate 2-hydroxylase	polymer	324	37186.1	1	UniProt (B1IVJ9)	Escherichia coli	G-2-H
2	B (D)	DNA (5'-P*AP*GP*CP*T-3')	polymer	4	1190.8	1		synthetic construct
3	C, D, E (A)	AMMONIUM ION	non-polymer		18.0	3	Chemie (NH4) PubChem (223)
4	F (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL) PubChem (753)
5	G (A)	FE (II) ION	non-polymer		55.8	1	Chemie (FE2) PubChem (27284)
6	H, I (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
7	J (A)	water	water		18.0	298	Chemie (HOH)

Sequence modifications

A: 1 - 320 (UniProt: B1IVJ9)

PDB	External Database	Details
Thr 24	Ile 24	conflict
Ala 182	Asn 182	engineered mutation
Cys 184	Gln 184	engineered mutation
Ala 270	Lys 270	engineered mutation
His 321	-	expression tag
His 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	38377.0
Non-Polymers*	Number of molecules	7
	Total formula weight	394.2
All*	Total formula weight	38771.1

*Water molecules are not included.