9N8F
Cryo-EM structure of SUDV glycoprotein with modified HR1c (L579P) and HR2 stalk bound to CA45 Fab
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, H, L (A, C, B) | SUDV Gulu - GP1 | polymer | 470 | 51221.8 | 3 | UniProt (Q7T9D9) | Sudan ebolavirus (strain Human/Uganda/Gulu/2000) (SEBOV Sudan Ebola virus) | GP1,2,GP |
| 2 | B, E, I (D, E, F) | SUDV Gulu - GP2 | polymer | 165 | 18572.0 | 3 | UniProt (Q7T9D9) | Sudan ebolavirus (strain Human/Uganda/Gulu/2000) (SEBOV Sudan Ebola virus) | GP1,2-delta |
| 3 | C, F, J (H, I, J) | CA45 Fab heavy chain | polymer | 236 | 25041.1 | 3 | Homo sapiens (human) | ||
| 4 | D, G, K (L, M, N) | CA45 Fab light chain | polymer | 215 | 23291.8 | 3 | Homo sapiens (human) | ||
| 5 | M, N, O (R, S, T) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 6 | P, Q, R (A, C, B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 3 | Chemie (NAG) |
Sequence modifications
D, E, F: 502 - 637 (UniProt: Q7T9D9)
| PDB | External Database | Details |
|---|---|---|
| Pro 579 | Leu 579 | engineered mutation |
| Leu 615 | Trp 615 | conflict |
| Ala 638 | - | expression tag |
| Ser 639 | - | expression tag |
| Gly 640 | - | expression tag |
| Tyr 641 | - | expression tag |
| Ile 642 | - | expression tag |
| Pro 643 | - | expression tag |
| Glu 644 | - | expression tag |
| Ala 645 | - | expression tag |
| Pro 646 | - | expression tag |
| Arg 647 | - | expression tag |
| Asp 648 | - | expression tag |
| Gly 649 | - | expression tag |
| Gln 650 | - | expression tag |
| Ala 651 | - | expression tag |
| Tyr 652 | - | expression tag |
| Val 653 | - | expression tag |
| Arg 654 | - | expression tag |
| Lys 655 | - | expression tag |
| Asp 656 | - | expression tag |
| Gly 657 | - | expression tag |
| Glu 658 | - | expression tag |
| Trp 659 | - | expression tag |
| Val 660 | - | expression tag |
| Leu 661 | - | expression tag |
| Leu 662 | - | expression tag |
| Ser 663 | - | expression tag |
| Thr 664 | - | expression tag |
| Phe 665 | - | expression tag |
| Leu 666 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 354379.8 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1273.2 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 663.6 | |
| All* | Total formula weight | 356316.6 |
