9N85

Cryo-EM structure of human importin beta:Ran-GTP:RanBP1 trimeric complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Importin subunit beta-1	polymer	876	97323.9	1	UniProt (Q14974) Pfam (PF03810) Pfam (PF00514) Pfam (PF25574)	Homo sapiens (human)	Importin-90,Karyopherin subunit beta-1,Nuclear factor p97,Pore targeting complex 97 kDa subunit,PTAC97
2	B (B)	Ran-specific GTPase-activating protein	polymer	144	16960.4	1	UniProt (P43487) Pfam (PF00638)	Homo sapiens (human)	Ran-binding protein 1,RanBP1
3	C (C)	GTP-binding nuclear protein Ran	polymer	210	23810.4	1	UniProt (P62826) Pfam (PF00071)	Homo sapiens (human)	Androgen receptor-associated protein 24,GTPase Ran,Ras-like protein TC4,Ras-related nuclear protein
4	D (C)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
5	E (C)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A: 1 - 876 (UniProt: Q14974)

PDB	External Database	Details
His 97	Gln 97	conflict
Arg 863	Thr 863	conflict

B: 17 - 160 (UniProt: P43487)

PDB	External Database	Details
His 17	Gln 26	conflict
Gln 19	Glu 28	conflict
Ala 20	Pro 29	conflict
Val 21	Ile 30	conflict
Pro 23	Ser 32	conflict
Ala 24	Leu 33	conflict
Asp 26	-	insertion
Ala 31	Lys 39	conflict
Cys 42	Lys 50	conflict
Asn 43	Met 51	conflict

C: 6 - 213 (UniProt: P62826)

PDB	External Database	Details
Glu 211	Asp 211	conflict
Ala 214	-	expression tag
Ala 215	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	138094.7
Non-Polymers*	Number of molecules	2
	Total formula weight	547.5
All*	Total formula weight	138642.2

*Water molecules are not included.