9N3Z
Type IV-A1 CRISPR effector complex bound to dsDNA and CasDinG - locked state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR type AFERR-associated protein Csf1 (Cas8) | polymer | 241 | 27036.0 | 1 | UniProt (A0AAB0B1U3) | Pseudomonas aeruginosa | Csf1 |
| 2 | B (B) | CCRISPR type AFERR-associated protein Csf3 (Cas5) | polymer | 220 | 24422.0 | 1 | UniProt (A0AAB0B1V2) | Pseudomonas aeruginosa | Csf3 |
| 3 | C, D, E, F, G (C, D, E, F, G) | CRISPR type AFERR-associated protein Csf2 (Cas7) | polymer | 359 | 38623.7 | 5 | UniProt (A0AAB0B1V3) | Pseudomonas aeruginosa | Csf2 |
| 4 | H (H) | CRISPR type AFERR-associated protein Csf5 (Cas6) | polymer | 232 | 25398.4 | 1 | UniProt (A0AAB0B1V7) | Pseudomonas aeruginosa | Csf5 |
| 5 | I (I) | crRNA (61-MER) | polymer | 61 | 19837.9 | 1 | Pseudomonas aeruginosa | ||
| 6 | J (J) | DNA, non-target strand (47-MER) | polymer | 47 | 14603.4 | 1 | synthetic construct | ||
| 7 | K (K) | DNA, target strand (47-MER) | polymer | 47 | 14349.2 | 1 | synthetic construct | ||
| 8 | L (L) | CRISPR-associated DEAD/DEAH-box helicase CasDinG | polymer | 748 | 81111.8 | 1 | UniProt (A0AA82WPF0) | Pseudomonas aeruginosa | CasDinG |
| 9 | M (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) PubChem (32051) |
Sequence modifications
C, D, E, F, G: 1 - 348 (UniProt: A0AAB0B1V3)
| PDB | External Database | Details |
|---|---|---|
| Met -10 | - | initiating methionine |
| Trp -9 | - | expression tag |
| Ser -8 | - | expression tag |
| His -7 | - | expression tag |
| Pro -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Phe -4 | - | expression tag |
| Glu -3 | - | expression tag |
| Lys -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 399877.1 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 65.4 | |
| All* | Total formula weight | 399942.5 |
