9N3Q

Crystal structure of PRMT5:MEP50 in complex with MTA and oxamide compound 51

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Protein arginine N-methyltransferase 5	polymer	645	73763.6	2	UniProt (O14744) Pfam (PF17285) Pfam (PF05185) Pfam (PF17286)	Homo sapiens (human)	72 kDa ICln-binding protein,Histone-arginine N-methyltransferase PRMT5,Jak-binding protein 1,Shk1 kinase-binding protein 1 homolog,SKB1Hs
2	B, D (B, D)	Methylosome protein 50	polymer	350	37862.4	2	UniProt (Q9BQA1) Pfam (PF00400)	Homo sapiens (human)	MEP-50,Androgen receptor cofactor p44,WD repeat-containing protein 77,Methylosome protein WDR77,p44/Mep50
3	E, G (A, C)	5'-DEOXY-5'-METHYLTHIOADENOSINE	non-polymer		297.3	2	Chemie (MTA)
4	F, H (A, C)	N-(6-amino-5-methylpyridin-3-yl)-2-{(2R,5S)-5-methyl-2-[2-(1-methylpiperidin-4-yl)-1,3-benzothiazol-5-yl]piperidin-1-yl}-2-oxoacetamide	non-polymer		506.7	2	Chemie (A1BV2)
5	I (C)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
6	J, K, L, M (A, B, C, D)	water	water		18.0	83	Chemie (HOH)

Sequence modifications

A, C: 2 - 637 (UniProt: O14744)

PDB	External Database	Details
Met -7	-	initiating methionine
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Lys 1	-	expression tag

B, D: 2 - 342 (UniProt: Q9BQA1)

PDB	External Database	Details
Met -7	-	initiating methionine
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	223252.1
Non-Polymers*	Number of molecules	5
	Total formula weight	1670.1
All*	Total formula weight	224922.1

*Water molecules are not included.