9N2U

Glutarate L-2-hydroxylase N187C mutant-5'-Mal-C6-GC DNA conjugate

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Glutarate 2-hydroxylase	polymer	324	37301.3	2	UniProt (B1IVJ9)	Escherichia coli	G-2-H
2	C, E (A, B)	(1r,4r)-4-[(2,5-dioxopyrrolidin-1-yl)methyl]cyclohexane-1-carboxamide	non-polymer		238.3	2	Chemie (A1BVO)
3	D, F (A, B)	FE (II) ION	non-polymer		55.8	2	Chemie (FE2)
4	G, H (A, B)	water	water		18.0	146	Chemie (HOH)

Sequence modifications

A, B: 1 - 320 (UniProt: B1IVJ9)

PDB	External Database	Details
Thr 24	Ile 24	conflict
Cys 187	Asn 187	engineered mutation
His 321	-	expression tag
His 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	74602.6
Non-Polymers*	Number of molecules	4
	Total formula weight	588.3
All*	Total formula weight	75190.8

*Water molecules are not included.