9N2B
Impacts of ribosomal RNA sequence variation on gene expression and phenotype: Cryo-EM structure of the rrsB ribosome (BBB-70S)
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (16) | 16S ribosomal RNA (rRNA) from the rrnB operon | polymer | 1542 | 499873.4 | 1 | GenBank (1789840096) | Escherichia coli K-12 | |
| 2 | B (SB) | 30S ribosomal protein S2 | polymer | 241 | 26781.7 | 1 | UniProt (P0A7V0) Pfam (PF00318) | Escherichia coli K-12 | Small ribosomal subunit protein uS2 |
| 3 | C (SC) | 30S ribosomal protein S3 | polymer | 233 | 26031.3 | 1 | UniProt (P0A7V3) Pfam (PF07650) Pfam (PF00189) | Escherichia coli K-12 | Small ribosomal subunit protein uS3 |
| 4 | D (SD) | 30S ribosomal protein S4 | polymer | 206 | 23514.2 | 1 | UniProt (P0A7V8) Pfam (PF00163) Pfam (PF01479) | Escherichia coli K-12 | Small ribosomal subunit protein uS4 |
| 5 | E (SE) | 30S ribosomal protein S5 | polymer | 167 | 17629.4 | 1 | UniProt (P0A7W1) Pfam (PF00333) Pfam (PF03719) | Escherichia coli K-12 | Small ribosomal subunit protein uS5 |
| 6 | F (SF) | 30S ribosomal protein S6 | polymer | 135 | 15727.5 | 1 | UniProt (P02358) Pfam (PF01250) | Escherichia coli K-12 | Small ribosomal subunit protein bS6 |
| 7 | G (SG) | 30S ribosomal protein S7 | polymer | 179 | 20055.2 | 1 | UniProt (P02359) Pfam (PF00177) | Escherichia coli K-12 | Small ribosomal subunit protein uS7 |
| 8 | H (SH) | 30S ribosomal protein S8 | polymer | 130 | 14146.6 | 1 | UniProt (P0A7W7) Pfam (PF00410) | Escherichia coli K-12 | Small ribosomal subunit protein uS8 |
| 9 | I (SI) | 30S ribosomal protein S9 | polymer | 130 | 14886.3 | 1 | UniProt (P0A7X3) Pfam (PF00380) | Escherichia coli K-12 | Small ribosomal subunit protein uS9 |
| 10 | J (SJ) | 30S ribosomal protein S10 | polymer | 103 | 11755.6 | 1 | UniProt (P0A7R5) Pfam (PF00338) | Escherichia coli K-12 | Small ribosomal subunit protein uS10 |
| 11 | K (SK) | Small ribosomal subunit protein uS11 | polymer | 129 | 13872.0 | 1 | UniProt (P0A7R9) Pfam (PF00411) | Escherichia coli K-12 | 30S ribosomal protein S11 |
| 12 | L (SL) | Small ribosomal subunit protein uS12 | polymer | 124 | 13814.2 | 1 | UniProt (P0A7S3) Pfam (PF00164) | Escherichia coli K-12 | 30S ribosomal protein S12 |
| 13 | M (SM) | 30S ribosomal protein S13 | polymer | 118 | 13128.5 | 1 | UniProt (P0A7S9) Pfam (PF00416) | Escherichia coli K-12 | Small ribosomal subunit protein uS13 |
| 14 | N (SN) | 30S ribosomal protein S14 | polymer | 101 | 11606.6 | 1 | UniProt (P0AG59) Pfam (PF00253) | Escherichia coli K-12 | Small ribosomal subunit protein uS14 |
| 15 | O (SO) | 30S ribosomal protein S15 | polymer | 89 | 10290.8 | 1 | UniProt (P0ADZ4) Pfam (PF00312) | Escherichia coli K-12 | Small ribosomal subunit protein uS15 |
| 16 | P (SP) | 30S ribosomal protein S16 | polymer | 82 | 9207.6 | 1 | UniProt (P0A7T3) Pfam (PF00886) | Escherichia coli K-12 | Small ribosomal subunit protein bS16 |
| 17 | Q (SQ) | 30S ribosomal protein S17 | polymer | 84 | 9724.5 | 1 | UniProt (P0AG63) Pfam (PF00366) | Escherichia coli K-12 | Small ribosomal subunit protein uS17 |
| 18 | R (SR) | 30S ribosomal protein S18 | polymer | 75 | 9005.5 | 1 | UniProt (P0A7T7) Pfam (PF01084) | Escherichia coli K-12 | |
| 19 | S (SS) | Small ribosomal subunit protein uS19 | polymer | 91 | 10326.2 | 1 | UniProt (P0A7U3) Pfam (PF00203) | Escherichia coli K-12 | 30S ribosomal protein S19 |
| 20 | T (ST) | 30S ribosomal protein S20 | polymer | 87 | 9708.5 | 1 | UniProt (P0A7U7) Pfam (PF01649) | Escherichia coli K-12 | Small ribosomal subunit protein bS20 |
| 21 | U (SU) | 30S ribosomal protein S21 | polymer | 71 | 8524.0 | 1 | UniProt (P68679) Pfam (PF01165) | Escherichia coli K-12 | Small ribosomal subunit protein bS21 |
| 22 | V (mR) | Synthetic messenger RNA (mRNA) 60 bp in length | polymer | 60 | 19128.4 | 1 | Escherichia coli BL21 | ||
| 23 | W (23) | 23S ribosomal RNA (rRNA) from the rrnB operon | polymer | 2904 | 941811.6 | 1 | Escherichia coli K-12 | ||
| 24 | X (5) | 5S ribosomal RNA (rRNA) from the B operon | polymer | 120 | 38814.1 | 1 | GenBank (1789840096) | Escherichia coli K-12 | |
| 25 | Y (LB) | 50S ribosomal protein L2 | polymer | 273 | 29923.6 | 1 | UniProt (P60422) Pfam (PF00181) Pfam (PF03947) | Escherichia coli K-12 | |
| 26 | Z (LC) | 50S ribosomal protein L3 | polymer | 209 | 22277.5 | 1 | UniProt (P60438) Pfam (PF00297) | Escherichia coli K-12 | Large ribosomal subunit protein uL3 |
| 27 | AA (LD) | Large ribosomal subunit protein uL4 | polymer | 201 | 22121.6 | 1 | UniProt (P60723) Pfam (PF00573) | Escherichia coli K-12 | 50S ribosomal protein L4 |
| 28 | BA (LE) | 50S ribosomal protein L5 | polymer | 179 | 20333.6 | 1 | UniProt (P62399) Pfam (PF00281) Pfam (PF00673) | Escherichia coli K-12 | |
| 29 | CA (LF) | 50S ribosomal protein L6 | polymer | 177 | 18932.8 | 1 | UniProt (P0AG55) Pfam (PF00347) | Escherichia coli K-12 | Large ribosomal subunit protein uL6 |
| 30 | DA (LI) | 50S ribosomal protein L9 | polymer | 149 | 15789.0 | 1 | UniProt (P0A7R1) Pfam (PF03948) Pfam (PF01281) | Escherichia coli K-12 | Large ribosomal subunit protein bL9 |
| 31 | EA (LM) | 50S ribosomal protein L13 | polymer | 142 | 16050.6 | 1 | UniProt (P0AA10) Pfam (PF00572) | Escherichia coli K-12 | Large ribosomal subunit protein uL13 |
| 32 | FA (LN) | 50S ribosomal protein L14 | polymer | 123 | 13565.1 | 1 | UniProt (P0ADY3) Pfam (PF00238) | Escherichia coli K-12 | Large ribosomal subunit protein uL14 |
| 33 | GA (LO) | 50S ribosomal protein L15 | polymer | 144 | 15008.5 | 1 | UniProt (P02413) Pfam (PF00828) | Escherichia coli K-12 | Large ribosomal subunit protein uL15 |
| 34 | HA (LP) | 50S ribosomal protein L16 | polymer | 136 | 15327.3 | 1 | UniProt (P0ADY7) Pfam (PF00252) | Escherichia coli K-12 | |
| 35 | IA (LQ) | 50S ribosomal protein L17 | polymer | 127 | 14393.7 | 1 | UniProt (P0AG44) Pfam (PF01196) | Escherichia coli K-12 | Large ribosomal subunit protein bL17 |
| 36 | JA (LR) | 50S ribosomal protein L18 | polymer | 117 | 12794.7 | 1 | UniProt (P0C018) Pfam (PF00861) | Escherichia coli K-12 | Large ribosomal subunit protein uL18 |
| 37 | KA (LS) | 50S ribosomal protein L19 | polymer | 115 | 13159.3 | 1 | UniProt (P0A7K6) Pfam (PF01245) | Escherichia coli K-12 | Large ribosomal subunit protein bL19 |
| 38 | LA (LT) | 50S ribosomal protein L20 | polymer | 118 | 13528.0 | 1 | UniProt (P0A7L3) Pfam (PF00453) | Escherichia coli K-12 | Large ribosomal subunit protein bL20 |
| 39 | MA (LU) | 50S ribosomal protein L21 | polymer | 103 | 11586.4 | 1 | UniProt (P0AG48) Pfam (PF00829) | Escherichia coli K-12 | Large ribosomal subunit protein bL21 |
| 40 | NA (LV) | Large ribosomal subunit protein uL22 | polymer | 110 | 12253.4 | 1 | UniProt (P61175) Pfam (PF00237) | Escherichia coli K-12 | 50S ribosomal protein L22 |
| 41 | OA (LW) | 50S ribosomal protein L23 | polymer | 100 | 11222.2 | 1 | UniProt (P0ADZ0) Pfam (PF00276) | Escherichia coli K-12 | Large ribosomal subunit protein uL23 |
| 42 | PA (LX) | 50S ribosomal protein L24 | polymer | 104 | 11339.3 | 1 | UniProt (P60624) Pfam (PF00467) Pfam (PF17136) | Escherichia coli K-12 | Large ribosomal subunit protein uL24 |
| 43 | QA (LY) | 50S ribosomal protein L25 | polymer | 94 | 10713.5 | 1 | UniProt (P68919) Pfam (PF01386) | Escherichia coli K-12 | Large ribosomal subunit protein bL25 |
| 44 | RA (La) | 50S ribosomal protein L27 | polymer | 85 | 9146.5 | 1 | UniProt (P0A7L8) Pfam (PF01016) | Escherichia coli K-12 | Large ribosomal subunit protein bL27 |
| 45 | SA (Lb) | 50S ribosomal protein L28 | polymer | 78 | 9027.6 | 1 | UniProt (P0A7M2) Pfam (PF00830) | Escherichia coli K-12 | Large ribosomal subunit protein bL28 |
| 46 | TA (Lc) | 50S ribosomal protein L29 | polymer | 63 | 7286.5 | 1 | UniProt (P0A7M6) Pfam (PF00831) | Escherichia coli K-12 | Large ribosomal subunit protein uL29 |
| 47 | UA (Ld) | 50S ribosomal protein L30 | polymer | 59 | 6554.8 | 1 | UniProt (P0AG51) Pfam (PF00327) | Escherichia coli K-12 | Large ribosomal subunit protein uL30 |
| 48 | VA (Le) | 50S ribosomal protein L31 | polymer | 70 | 7887.1 | 1 | UniProt (P0A7M9) Pfam (PF01197) | Escherichia coli K-12 | Large ribosomal subunit protein bL31-A |
| 49 | WA (Lf) | 50S ribosomal protein L32 | polymer | 57 | 6463.4 | 1 | UniProt (P0A7N4) Pfam (PF01783) | Escherichia coli K-12 | Large ribosomal subunit protein bL32 |
| 50 | XA (Lg) | 50S ribosomal protein L33 | polymer | 55 | 6388.6 | 1 | UniProt (P0A7N9) Pfam (PF00471) | Escherichia coli K-12 | |
| 51 | YA (Lh) | 50S ribosomal protein L34 | polymer | 46 | 5397.5 | 1 | UniProt (P0A7P5) Pfam (PF00468) | Escherichia coli K-12 | Large ribosomal subunit protein bL34 |
| 52 | ZA (Li) | 50S ribosomal protein L35 | polymer | 65 | 7313.0 | 1 | UniProt (P0A7Q1) Pfam (PF01632) | Escherichia coli K-12 | Large ribosomal subunit protein bL35,Ribosomal protein A |
| 53 | AB (Lj) | 50S ribosomal protein L36 | polymer | 38 | 4377.4 | 1 | UniProt (P0A7Q6) Pfam (PF00444) | Escherichia coli K-12 | Large ribosomal subunit protein bL36-A,Ribosomal protein B |
| 54 | BB (Pt) | N-formyl-methionine initiator tRNA (fMet-tRNAfMet) | polymer | 77 | 24848.9 | 1 | GenBank (2696706521) | Escherichia coli K-12 | |
| 55 | CB, PE, QE, RE, SE... (16, 23) | 1,4-DIAMINOBUTANE | non-polymer | 88.2 | 9 | Chemie (PUT) | |||
| 56 | AF, DB, XE, YE, ZE (23, 16) | SPERMIDINE | non-polymer | 145.2 | 5 | Chemie (SPD) | |||
| 57 | AC, AD, AE, AG, AH... (16, 23, 5, LB, LC...) | MAGNESIUM ION | non-polymer | 24.3 | 276 | Chemie (MG) | |||
| 58 | AN, BE, BN, CE, CN... (23, 16, LB, LX) | POTASSIUM ION | non-polymer | 39.1 | 56 | Chemie (K) | |||
| 59 | JE, KO, MO (SB, Le, Lj) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) | |||
| 60 | NE, OE (23) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (ATP) | |||
| 61 | NO (Pt) | N-FORMYLMETHIONINE | non-polymer | 177.2 | 1 | Chemie (FME) |
Sequence modifications
SK: 1 - 129 (UniProt: P0A7R9)
| PDB | External Database | Details |
|---|---|---|
| Ias 119 | Asn 119 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 54 |
| Total formula weight | 2184374.6 | |
| Non-Polymers* | Number of molecules | 352 |
| Total formula weight | 11805.1 | |
| All* | Total formula weight | 2196179.7 |
