9N29
hM4Di(GRANPA) in complex with mGsI and diphenhydramine
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 351 | 38744.4 | 1 | UniProt (P54311) | Rattus norvegicus (Norway rat) | Transducin beta chain 1 |
| 2 | B (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 68 | 7563.8 | 1 | UniProt (P59768) | Homo sapiens (human) | G gamma-I |
| 3 | C (N) | nanobody 35 | polymer | 138 | 15140.7 | 1 | Lama glama (llama) | ||
| 4 | D (A) | Guanine nucleotide-binding protein Gi chimera (mGsI) | polymer | 245 | 27915.7 | 1 | Homo sapiens (human) | ||
| 5 | E (R) | Muscarinic acetylcholine receptor M4 | polymer | 478 | 52896.8 | 1 | UniProt (P08173) | Homo sapiens (human) | |
| 6 | F (R) | N-[2-(BENZHYDRYLOXY)ETHYL]-N,N-DIMETHYLAMINE | non-polymer | 255.4 | 1 | Chemie (2PM) PubChem (3100) |
Sequence modifications
B: 2 - 340 (UniProt: P54311)
R: 2 - 479 (UniProt: P08173)
| PDB | External Database | Details |
|---|---|---|
| Met -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Val 85 | Ser 85 | engineered mutation |
| Cys 113 | Tyr 113 | engineered mutation |
| Gly 203 | Ala 203 | engineered mutation |
| Phe 416 | Tyr 416 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 142261.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 255.4 | |
| All* | Total formula weight | 142516.7 |
