9N04
Cryo-EM structure of GCGR-Gs complex with peptide 15
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45699.4 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 530 | 57962.4 | 1 | Homo sapiens (human) | ||
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 58 | 6375.3 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (N) | Nb35 | polymer | 128 | 13885.4 | 1 | Lama glama (llama) | ||
| 5 | E (R) | Glucagon receptor | polymer | 475 | 54000.4 | 1 | UniProt (P47871) Pfam (PF02793) Pfam (PF00002) | Homo sapiens (human) | GL-R |
| 6 | F (P) | Peptide15: Dual agonist peptide | polymer | 30 | 3383.7 | 1 | synthetic construct | ||
| 7 | G, H, I, J (A, B, R, P) | water | water | 18.0 | 31 | Chemie (HOH) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
R: 26 - 477 (UniProt: P47871)
| PDB | External Database | Details |
|---|---|---|
| Asn 54 | Ser 54 | engineered mutation |
| Ala 226 | Gly 226 | engineered mutation |
| Ala 268 | Glu 268 | engineered mutation |
| Lys 271 | Asn 271 | engineered mutation |
| Asp 274 | Lys 274 | engineered mutation |
| Lys 280 | Arg 280 | engineered mutation |
| Asp 284 | Thr 284 | engineered mutation |
| Thr 285 | Ile 285 | engineered mutation |
| Ser 366 | Ala 366 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 10 | - | expression tag |
| Lys 11 | - | expression tag |
| Thr 12 | - | expression tag |
| Ile 13 | - | expression tag |
| Ile 14 | - | expression tag |
| Ala 15 | - | expression tag |
| Leu 16 | - | expression tag |
| Ser 17 | - | expression tag |
| Tyr 18 | - | expression tag |
| Ile 19 | - | expression tag |
| Phe 20 | - | expression tag |
| Cys 21 | - | expression tag |
| Leu 22 | - | expression tag |
| Val 23 | - | expression tag |
| Phe 24 | - | expression tag |
| Ala 25 | - | expression tag |
| Asp 478 | - | expression tag |
| Tyr 479 | - | expression tag |
| Lys 480 | - | expression tag |
| Asp 481 | - | expression tag |
| Asp 482 | - | expression tag |
| Asp 483 | - | expression tag |
| Asp 484 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 181306.7 | |
| All* | Total formula weight | 181306.7 |
