9MUJ

RlmR 23S rRNA methyltransferase from Thermus thermophilus in complex with methylated rRNA (Um2552) and S-adenosyl-L-homocysteine (SAH)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	23S rRNA methyltransferase	polymer	280	30239.7	2	UniProt (Q72GY4) Pfam (PF22435) Pfam (PF00588)	Thermus thermophilus HB27
2	C (C)	RNA (59-MER)	polymer	59	19105.4	1	GenBank (46197919)	Thermus thermophilus HB27
3	D (A)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
4	E (B)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
5	F, G, H (A, B, C)	water	water		18.0	422	Chemie (HOH)

Sequence modifications

A, B: 1 - 260 (UniProt: Q72GY4)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	79584.8
Non-Polymers*	Number of molecules	2
	Total formula weight	782.8
All*	Total formula weight	80367.7

*Water molecules are not included.