9MSH
de novo SigN RNA polymerase open complex (RPo)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (G, H) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (I) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (J) | DNA-directed RNA polymerase subunit beta' | polymer | 1415 | 156338.9 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (K) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (M) | RNA polymerase sigma-54 factor | polymer | 477 | 54043.5 | 1 | UniProt (P24255) Pfam (PF00309) Pfam (PF04963) Pfam (PF04552) | Escherichia coli | |
| 6 | G (U) | dhsU (-60 to +30) non-template strand | polymer | 90 | 28003.0 | 1 | Aquifex aeolicus VF5 | ||
| 7 | H (V) | dhsU (-60 to +30) template strand | polymer | 90 | 27507.6 | 1 | Aquifex aeolicus VF5 | ||
| 8 | I (I) | PYROPHOSPHATE 2- | non-polymer | 176.0 | 1 | Chemie (POP) | |||
| 9 | J (J) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 10 | K, L (J) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 11 | M, N, O, P, Q... (G, H, I, J, K...) | water | water | 18.0 | 348 | Chemie (HOH) |
Sequence modifications
J: 1 - 1407 (UniProt: P0A8T7)
| PDB | External Database | Details |
|---|---|---|
| Leu 1408 | - | expression tag |
| Glu 1409 | - | expression tag |
| Leu 1410 | - | expression tag |
| Glu 1411 | - | expression tag |
| Val 1412 | - | expression tag |
| Leu 1413 | - | expression tag |
| Phe 1414 | - | expression tag |
| Gln 1415 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 500080.8 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 331.1 | |
| All* | Total formula weight | 500411.9 |
