9MMP

Cryo-EM structure of CRAF/MEK1/14-3-3 complex (autoinhibited conformation)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (C, D)	14-3-3 protein zeta	polymer	247	28108.5	2	UniProt (V9P4T4) Pfam (PF00244) UniProt (by SIFTS) (Q2F637)	Spodoptera exigua (beet armyworm)
2	C (A)	RAF proto-oncogene serine/threonine-protein kinase	polymer	683	77414.0	1	UniProt (P04049) Pfam (PF02196) Pfam (PF00130) Pfam (PF00069)	Homo sapiens (human)	Proto-oncogene c-RAF,cRaf,Raf-1
3	D (B)	Dual specificity mitogen-activated protein kinase kinase 1	polymer	415	45934.5	1	UniProt (Q02750) Pfam (PF00069)	Homo sapiens (human)	MKK1,ERK activator kinase 1,MAPK/ERK kinase 1,MEK 1
4	E, H (A, B)	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	2	Chemie (AGS)
5	F, G (A)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
6	I (B)	5-[(2-fluoro-4-iodophenyl)amino]-N-(2-hydroxyethoxy)imidazo[1,5-a]pyridine-6-carboxamide	non-polymer		456.2	1	Chemie (LCJ)

Sequence modifications

A: 1 - 679 (UniProt: P04049)

PDB	External Database	Details
Met -21	-	initiating methionine
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ala -10	-	expression tag
Val -9	-	expression tag
Asp -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Gly 0	-	expression tag
Arg 156	Gln 156	engineered mutation
Glu 587	Asp 587	engineered mutation
Val 680	-	expression tag
Pro 681	-	expression tag
Ala 682	-	expression tag
Trp 683	-	expression tag
Ser 684	-	expression tag
His 685	-	expression tag
Pro 686	-	expression tag
Gln 687	-	expression tag
Phe 688	-	expression tag
Glu 689	-	expression tag
Lys 690	-	expression tag
His 691	-	expression tag
His 692	-	expression tag

B: 1 - 393 (UniProt: Q02750)

PDB	External Database	Details
Met -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ala -10	-	expression tag
Val -9	-	expression tag
Asp -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Gly 0	-	expression tag
Ala 218	Ser 218	engineered mutation
Ala 222	Ser 222	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	179565.6
Non-Polymers*	Number of molecules	5
	Total formula weight	1633.5
All*	Total formula weight	181199.1

*Water molecules are not included.