9MKU
FnoCas12a bridge helix variant state 2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | crRNA | polymer | 43 | 13749.1 | 1 | synthetic construct | ||
| 2 | B (C) | TS DNA | polymer | 24 | 7302.8 | 1 | synthetic construct | ||
| 3 | C (A) | CRISPR-associated endonuclease Cas12a | polymer | 1305 | 152515.5 | 1 | UniProt (A0Q7Q2) | Francisella tularensis subsp. novicida U112 | CRISPR-associated endonuclease Cpf1,FnCas12a,FnCpf1 |
Sequence modifications
A: 1 - 1300 (UniProt: A0Q7Q2)
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Ala -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Pro 969 | Lys 969 | engineered mutation |
| Pro 970 | Asp 970 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 173567.4 | |
| All* | Total formula weight | 173567.4 |
