9MII
253-7A03 Fab in complex with HIV-1 BG505 SOSIP Env trimer and RM20A3 Fab
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E, I (A, C, E) | HIV-1 Envelope Glycoprotein BG505 SOSIP.664 gp120 | polymer | 516 | 57946.0 | 3 | UniProt (Q2N0S6) Pfam (PF00516) | Human immunodeficiency virus 1 | |
| 2 | B, F, J (B, D, F) | Envelope glycoprotein gp160 | polymer | 153 | 17146.5 | 3 | UniProt (Q2N0S6) Pfam (PF00517) UniProt (by SIFTS) (D0PUM0) | Human immunodeficiency virus 1 | |
| 3 | C, G, K (G, J, M) | RM20A3 heavy chain Fv | polymer | 125 | 13511.1 | 3 | Macaca mulatta | ||
| 4 | D, H, L (I, K, N) | RM20A3 light chain Fv | polymer | 128 | 13508.8 | 3 | Macaca mulatta | ||
| 5 | M (H) | 253-7A03 heavy chain Fv | polymer | 119 | 13357.9 | 1 | Homo sapiens | ||
| 6 | N (L) | 253-7A03 lambda chain Fv | polymer | 100 | 10474.4 | 1 | Homo sapiens | ||
| 7 | O, P, Q, S, T (O, P, Q, S, T) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 5 | In PDB GlyTouCan (G42666HT) | |||
| 8 | R (R) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 1 | In PDB GlyTouCan (G15407YE) | |||
| 9 | U (U) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 1 | In PDB GlyTouCan (G81315DD) | |||
| 10 | V (V) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 11 | AA, AB, BA, BB, CA... (A, E, B, C, F...) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 44 | Chemie (NAG) |
Sequence modifications
A, C, E: 31 - 508 (UniProt: Q2N0S6)
B, D, F: 512 - 664 (UniProt: Q2N0S6)
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Met -1 | - | expression tag |
| Lys 0 | - | expression tag |
| Arg 1 | - | expression tag |
| Gly 2 | - | expression tag |
| Leu 3 | - | expression tag |
| Cys 4 | - | expression tag |
| Cys 5 | - | expression tag |
| Val 6 | - | expression tag |
| Leu 7 | - | expression tag |
| Leu 8 | - | expression tag |
| Leu 9 | - | expression tag |
| Cys 10 | - | expression tag |
| Gly 11 | - | expression tag |
| Ala 12 | - | expression tag |
| Val 13 | - | expression tag |
| Phe 14 | - | expression tag |
| Val 15 | - | expression tag |
| Ser 16 | - | expression tag |
| Pro 17 | - | expression tag |
| Ser 18 | - | expression tag |
| Gln 19 | - | expression tag |
| Glu 20 | - | expression tag |
| Ile 21 | - | expression tag |
| His 22 | - | expression tag |
| Ala 23 | - | expression tag |
| Arg 24 | - | expression tag |
| Phe 25 | - | expression tag |
| Arg 26 | - | expression tag |
| Arg 27 | - | expression tag |
| Gly 28 | - | expression tag |
| Ala 29 | - | expression tag |
| Arg 30 | - | expression tag |
| Asn 332 | Thr 330 | engineered mutation |
| Cys 501 | Ala 498 | engineered mutation |
| Arg 509 | - | expression tag |
| Arg 510 | - | expression tag |
| Arg 511 | - | expression tag |
| Arg 512 | - | expression tag |
| Arg 513 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Pro 559 | Ile 556 | conflict |
| Cys 605 | Thr 602 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 330169.5 | |
| Branched | Number of molecules | 8 |
| Total formula weight | 4368.1 | |
| Non-Polymers* | Number of molecules | 44 |
| Total formula weight | 9733.2 | |
| All* | Total formula weight | 344270.7 |
