9MGG

beta-barrel assembly machine from Escherichia coli in a late state of substrate assembly

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Outer membrane protein assembly factor BamA	polymer	798	89608.0	1	UniProt (P0A940) Pfam (PF07244) Pfam (PF01103)	Escherichia coli K-12	Omp85
2	B (C)	Outer membrane protein assembly factor BamC	polymer	344	36875.3	1	UniProt (P0A903) Pfam (PF06804)	Escherichia coli K-12
3	C (D)	Outer membrane protein assembly factor BamD	polymer	245	27858.3	1	UniProt (P0AC02) Pfam (PF13525)	Escherichia coli K-12
4	D (E)	Outer membrane protein assembly factor BamE	polymer	113	12311.0	1	UniProt (P0A937) Pfam (PF04355)	Escherichia coli K-12
5	E (B)	Outer membrane protein assembly factor BamB	polymer	392	41918.9	1	UniProt (P77774) Pfam (PF13360)	Escherichia coli K-12
6	F (F)	Outer membrane protein assembly factor BamA	polymer	568	62852.7	1	UniProt (P0A940) Pfam (PF07244) Pfam (PF01103)	Escherichia coli K-12	Omp85

Sequence modifications

A: 24 - 810 (UniProt: P0A940)

PDB	External Database	Details
Ala 13	-	expression tag
Glu 14	-	expression tag
Gly 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
Cys 512	Asp 512	engineered mutation

F: 272 - 421 (UniProt: P0A940)

PDB	External Database	Details
Ala 243	-	expression tag
Trp 244	-	expression tag
Ser 245	-	expression tag
His 246	-	expression tag
Pro 247	-	expression tag
Gln 248	-	expression tag
Phe 249	-	expression tag
Glu 250	-	expression tag
Lys 251	-	expression tag
Gly 252	-	expression tag
Gly 253	-	expression tag
Gly 254	-	expression tag
Ser 255	-	expression tag
Gly 256	-	expression tag
Gly 257	-	expression tag
Gly 258	-	expression tag
Ser 259	-	expression tag
Gly 260	-	expression tag
Gly 261	-	expression tag
Ser 262	-	expression tag
Ala 263	-	expression tag
Trp 264	-	expression tag
Ser 265	-	expression tag
His 266	-	expression tag
Pro 267	-	expression tag
Gln 268	-	expression tag
Phe 269	-	expression tag
Glu 270	-	expression tag
Lys 271	-	expression tag

F: 422 - 810 (UniProt: P0A940)

PDB	External Database	Details
Cys 458	Ile 458	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	271424.2
All*	Total formula weight	271424.2

*Water molecules are not included.