9MGF

beta-barrel assembly machine from Escherichia coli in a middle state of substrate assembly

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Outer membrane protein assembly factor BamA	polymer	790	88502.8	1	UniProt (P0A940) Pfam (PF07244) Pfam (PF01103)	Escherichia coli K-12	Omp85
2	B (B)	Outer membrane protein assembly factor BamB	polymer	373	39882.4	1	UniProt (P77774) Pfam (PF13360)	Escherichia coli K-12
3	C (C)	Outer membrane protein assembly factor BamC	polymer	320	34401.3	1	UniProt (P0A903) Pfam (PF06804)	Escherichia coli K-12
4	D (D)	Outer membrane protein assembly factor BamD	polymer	226	25816.8	1	UniProt (P0AC02) Pfam (PF13525)	Escherichia coli K-12
5	E (E)	Outer membrane protein assembly factor BamE	polymer	104	11610.8	1	UniProt (P0A937) Pfam (PF04355)	Escherichia coli K-12
6	F (F)	Outer membrane protein assembly factor BamA	polymer	568	62908.8	1	UniProt (P0A940) Pfam (PF07244) Pfam (PF01103)	Escherichia coli K-12	Omp85
7	G (G)	Unknown peptide	polymer	8	635.9	1		Escherichia coli K-12

Sequence modifications

A: 21 - 810 (UniProt: P0A940)

PDB	External Database	Details
Cys 512	Asp 512	engineered mutation

E: 20 - 113 (UniProt: P0A937)

PDB	External Database	Details
Gly 114	-	expression tag
Gly 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
His 120	-	expression tag
His 121	-	expression tag
His 122	-	expression tag
His 123	-	expression tag

F: 272 - 421 (UniProt: P0A940)

PDB	External Database	Details
Ala 243	-	expression tag
Trp 244	-	expression tag
Ser 245	-	expression tag
His 246	-	expression tag
Pro 247	-	expression tag
Gln 248	-	expression tag
Phe 249	-	expression tag
Glu 250	-	expression tag
Lys 251	-	expression tag
Gly 252	-	expression tag
Gly 253	-	expression tag
Gly 254	-	expression tag
Ser 255	-	expression tag
Gly 256	-	expression tag
Gly 257	-	expression tag
Gly 258	-	expression tag
Ser 259	-	expression tag
Gly 260	-	expression tag
Gly 261	-	expression tag
Ser 262	-	expression tag
Ala 263	-	expression tag
Trp 264	-	expression tag
Ser 265	-	expression tag
His 266	-	expression tag
Pro 267	-	expression tag
Gln 268	-	expression tag
Phe 269	-	expression tag
Glu 270	-	expression tag
Lys 271	-	expression tag

F: 422 - 810 (UniProt: P0A940)

PDB	External Database	Details
Cys 597	Gly 597	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	263758.8
All*	Total formula weight	263758.8

*Water molecules are not included.