9MG6
Structure of Saccharomyces cerevisiae mRNA cap (guanine-N7) methyltransferase variant, Abd1-K163R-K311R-F387Y-Y416F, in complex with sinefungin and GTP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | mRNA cap guanine-N7 methyltransferase | polymer | 296 | 34270.9 | 3 | UniProt (A6ZLH5) Pfam (PF03291) UniProt (by SIFTS) (P32783) | Saccharomyces cerevisiae YJM789 | |
| 2 | D, K, S (A, B, C) | SINEFUNGIN | non-polymer | 381.4 | 3 | Chemie (SFG) | |||
| 3 | E, L, T (A, B, C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 3 | Chemie (GTP) | |||
| 4 | F, M, N, U (A, B, C) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 4 | Chemie (EDO) | |||
| 5 | G, H, I, J, O... (A, B, C) | SULFATE ION | non-polymer | 96.1 | 13 | Chemie (SO4) | |||
| 6 | AA, BA, CA (A, B, C) | water | water | 18.0 | 240 | Chemie (HOH) |
Sequence modifications
A, B, C: 141 - 436 (UniProt: A6ZLH5)
| PDB | External Database | Details |
|---|---|---|
| Arg 163 | Lys 163 | engineered mutation |
| Arg 311 | Lys 311 | engineered mutation |
| Tyr 387 | Phe 387 | engineered mutation |
| Phe 416 | Tyr 416 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 102812.6 | |
| Non-Polymers* | Number of molecules | 23 |
| Total formula weight | 4210.8 | |
| All* | Total formula weight | 107023.4 |
