9MG6
Structure of Saccharomyces cerevisiae mRNA cap (guanine-N7) methyltransferase variant, Abd1-K163R-K311R-F387Y-Y416F, in complex with sinefungin and GTP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | mRNA cap guanine-N7 methyltransferase | polymer | 296 | 34270.9 | 3 | UniProt (A6ZLH5) Pfam (PF03291) UniProt (by SIFTS) (P32783) | Saccharomyces cerevisiae YJM789 | |
| 2 | D, K, S (A, B, C) | SINEFUNGIN | non-polymer | 381.4 | 3 | Chemie (SFG) PubChem (456189) PubChem (4065234) PubChem (6917665) PubChem (12771337) PubChem (25246236) PubChem (176612030) PubChem (177116271) PubChem (46875078) PubChem (46936257) PubChem (65482) PubChem (70789212) PubChem (93287274) PubChem (12771338) PubChem (70602121) PubChem (92403972) PubChem (93287270) PubChem (93287272) PubChem (93475589) PubChem (131971033) PubChem (131972810) PubChem (138626357) PubChem (156562738) PubChem (162866891) PubChem (163339130) PubChem (168280605) | |||
| 3 | E, L, T (A, B, C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 3 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 4 | F, M, N, U (A, B, C) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 4 | Chemie (EDO) | |||
| 5 | G, H, I, J, O... (A, B, C) | SULFATE ION | non-polymer | 96.1 | 13 | Chemie (SO4) | |||
| 6 | AA, BA, CA (A, B, C) | water | water | 18.0 | 240 | Chemie (HOH) |
Sequence modifications
A, B, C: 141 - 436 (UniProt: A6ZLH5)
| PDB | External Database | Details |
|---|---|---|
| Arg 163 | Lys 163 | engineered mutation |
| Arg 311 | Lys 311 | engineered mutation |
| Tyr 387 | Phe 387 | engineered mutation |
| Phe 416 | Tyr 416 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 102812.6 | |
| Non-Polymers* | Number of molecules | 23 |
| Total formula weight | 4210.8 | |
| All* | Total formula weight | 107023.4 |
