9M9E

Structural Basis of Pausing During Transcription Initiation in Mycobacterium tuberculosis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	347	37745.3	2	UniProt (P9WGZ1)	Mycobacterium tuberculosis H37Rv	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (C)	DNA-directed RNA polymerase subunit beta	polymer	1178	130018.8	1	UniProt (P9WGY9)	Mycobacterium tuberculosis H37Rv	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D (D)	DNA-directed RNA polymerase subunit beta'	polymer	1326	148350.4	1	UniProt (P9WGY7)	Mycobacterium tuberculosis H37Rv	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E (E)	DNA-directed RNA polymerase subunit omega	polymer	110	11851.1	1	UniProt (P9WGY5)	Mycobacterium tuberculosis H37Rv	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F (F)	ECF RNA polymerase sigma factor SigE	polymer	263	29740.1	1	UniProt (P9WGG7)	Mycobacterium tuberculosis H37Rv	ECF sigma factor SigE,Alternative RNA polymerase sigma factor SigE,RNA polymerase sigma-E factor,Sigma-E factor
6	G (G)	DNA (48-MER)	polymer	48	14797.5	1		Mycobacterium tuberculosis H37Rv
7	H (H)	DNA (48-MER)	polymer	48	14823.5	1		Mycobacterium tuberculosis H37Rv
8	I (I)	RNA (5'-R(*CP*CP*CP*UP*CP*GP*A)-3')	polymer	7	2156.3	1		Mycobacterium tuberculosis H37Rv
9	J (M)	RNA polymerase-binding transcription factor CarD	polymer	169	18819.3	1	UniProt (P9WJG3)	Mycobacterium tuberculosis H37Rv
10	K (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
11	L, M (D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

D: 1 - 1316 (UniProt: P9WGY7)

PDB	External Database	Details
His 1317	-	expression tag
His 1318	-	expression tag
His 1319	-	expression tag
His 1320	-	expression tag
His 1321	-	expression tag
His 1322	-	expression tag
His 1323	-	expression tag
His 1324	-	expression tag
His 1325	-	expression tag
His 1326	-	expression tag

F: 2 - 257 (UniProt: P9WGG7)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag

M: 2 - 162 (UniProt: P9WJG3)

PDB	External Database	Details
Met -6	-	initiating methionine
Gly -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	446047.8
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	446202.9

*Water molecules are not included.