9M8D

Cryo-EM structure of the dihydrolipoamide dehydrogenase complexed with the lipoyllysine and binding domain of the dihydrolipoyl transacetylase from Mycobacterium smegmatis pyruvate dehydrogenase complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Dihydrolipoyl dehydrogenase	polymer	464	49525.3	2	UniProt (A0QQW8) Pfam (PF07992) Pfam (PF02852)	Mycolicibacterium smegmatis MC2 155
2	C (C)	Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex	polymer	595	62473.7	1	UniProt (A0R072) Pfam (PF00364) Pfam (PF02817) Pfam (PF00198)	Mycolicibacterium smegmatis MC2 155
3	D, E (A, B)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
4	F, G (C)	DIHYDROLIPOIC ACID	non-polymer		208.3	2	Chemie (RED) PubChem (9834298)	Mycolicibacterium smegmatis MC2 155

Sequence modifications

C: 20 - 585 (UniProt: A0R072)

PDB	External Database	Details
His 586	-	expression tag
His 587	-	expression tag
His 588	-	expression tag
His 589	-	expression tag
His 590	-	expression tag
His 591	-	expression tag
His 592	-	expression tag
His 593	-	expression tag
His 594	-	expression tag
His 595	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	161524.3
Non-Polymers*	Number of molecules	4
	Total formula weight	1987.8
All*	Total formula weight	163512.1

*Water molecules are not included.