9M7O
Cryo-EM structure of Ufd2/Ubc4-ub complex with K29triUb(monomeric conformation)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A (B) | Ubiquitin-conjugating enzyme E2 4 | polymer | 148 | 16442.6 | 1 | UniProt (P15731) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (brewer's yeast) | E2 ubiquitin-conjugating enzyme 4,Ubiquitin carrier protein 4,Ubiquitin-protein ligase 4 |
2 | B (D) | Polyubiquitin-C | polymer | 77 | 8665.9 | 1 | UniProt (P0CG48) | Homo sapiens (human) | Proximal Ub of K29tirUb |
3 | C (E) | Polyubiquitin-C | polymer | 76 | 8604.8 | 1 | UniProt (P0CG48) | Homo sapiens (human) | Distal Ub of K29tirUb |
4 | D (C) | Ubiquitin | polymer | 76 | 8576.8 | 1 | UniProt (P62979) | Homo sapiens (human) | Donor Ub |
5 | E (A) | E4 ubiquitin-protein ligase UFD2 | polymer | 961 | 110044.5 | 1 | UniProt (P54860) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (brewer's yeast) | RING-type E3 ubiquitin transferase UFD2,Ubiquitin conjugation factor E4,Ubiquitin fusion degradation protein 2,UB fusion protein 2 |
Sequence modifications
B: 1 - 148 (UniProt: P15731)
D: 1 - 76 (UniProt: P0CG48)
E: 1 - 76 (UniProt: P0CG48)
PDB | External Database | Details |
---|---|---|
Ser 22 | Cys 22 | engineered mutation |
Ser 108 | Cys 108 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Cys 48 | Lys 48 | engineered mutation |
Asp 77 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Arg 29 | Lys 29 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 152334.7 | |
All* | Total formula weight | 152334.7 |