9M7G
Cryo-EM structure of the human glucagon receptor (GCGR) in ligand free state resolved via the fusion/crosslinking strategy
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Glucagon receptor | polymer | 476 | 53980.3 | 1 | UniProt (P47871) Pfam (PF02793) Pfam (PF00002) | Homo sapiens (human) | GL-R |
| 2 | B (B) | Green fluorescent protein | polymer | 196 | 22388.3 | 1 | UniProt (A0A059PIQ0) | Aequorea victoria | |
| 3 | C (C) | GFP-clamp E186C | polymer | 294 | 31038.7 | 1 | synthetic construct |
Sequence modifications
A: 26 - 329 (UniProt: P47871)
A: 345 - 477 (UniProt: P47871)
B: 3 - 197 (UniProt: A0A059PIQ0)
| PDB | External Database | Details |
|---|---|---|
| Cys 257 | Thr 257 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Arg 1001 | - | linker |
| Arg 1002 | - | linker |
| Gln 1003 | - | linker |
| Leu 1004 | - | linker |
| Gly 1005 | - | linker |
| His 1006 | - | linker |
| Tyr 1007 | - | linker |
| Leu 1008 | - | linker |
| Ser 1009 | - | linker |
| Thr 1010 | - | linker |
| Gln 1011 | - | linker |
| Thr 1012 | - | linker |
| Ser 1013 | - | linker |
| Leu 1014 | - | linker |
| Ser 1015 | - | linker |
| Lys 1016 | - | linker |
| Asp 1017 | - | linker |
| Leu 1018 | - | linker |
| Asn 1019 | - | linker |
| Glu 1020 | - | linker |
| Lys 1021 | - | linker |
| Arg 1022 | - | linker |
| Asp 1023 | - | linker |
| His 1024 | - | linker |
| Met 1025 | - | linker |
| Val 1026 | - | linker |
| Leu 1027 | - | linker |
| Leu 1028 | - | linker |
| Glu 1029 | - | linker |
| Phe 1030 | - | linker |
| Val 1031 | - | linker |
| Thr 1032 | - | linker |
| Ala 1033 | - | linker |
| Ala 1034 | - | linker |
| Gly 1035 | - | linker |
| Leu 1036 | - | linker |
| Gln 1037 | - | linker |
| Lys 1038 | - | linker |
| Glu 1039 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Gly 1 | - | expression tag |
| Thr 2 | - | expression tag |
| Ile 17 | Val 16 | conflict |
| Arg 31 | Ser 30 | conflict |
| Tyr 40 | Asn 39 | conflict |
| Cro 67 | Thr 65 | conflict |
| - | Tyr 66 | deletion |
| - | Gly 67 | deletion |
| Ser 73 | Ala 72 | conflict |
| Arg 81 | Gln 80 | conflict |
| Tyr 100 | Ser 99 | conflict |
| Met 154 | Thr 153 | conflict |
| Asn 159 | Lys 158 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 107407.2 | |
| All* | Total formula weight | 107407.2 |
