9M74

Crystal structure of MBP-fused BIL1/BZR1 (21-104) in complex with double-stranded DNA contaning CACAGCTGTG

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E, F (C, D, A, B)	Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1	polymer	453	49819.4	4	UniProt (A0A4P1LXE0) UniProt (Q8S307) Pfam (PF05687)	Arabidopsis thaliana (thale cress)	Protein BIN2 SUBSTRATE 2
2	C, D, G, H (G, H, E, F)	DNA (5'-D(*TP*TP*CP*AP*CP*AP*GP*CP*TP*GP*TP*GP*AP*AP*A)-3')	polymer	15	4593.0	4		synthetic construct
3	I, J, K, L (J, K, L, M)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	4	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)
4	M, N, O (C, A, B)	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
5	P, Q, R, S, T... (C, D, G, H, A...)	water	water		18.0	185	Chemie (HOH)

Sequence modifications

C, D, A, B: -347 - 21 (UniProt: A0A4P1LXE0)

PDB	External Database	Details
Met -348	-	initiating methionine
Ala -266	Asp 84	engineered mutation
Ala -265	Lys 85	engineered mutation
Ala -176	Glu 174	engineered mutation
Ala -175	Asn 175	engineered mutation
Ala -109	Lys 241	engineered mutation
Ala 11	Glu 361	engineered mutation
Ala 14	Lys 364	engineered mutation
Ala 15	Asp 365	engineered mutation

C, D, A, B: 22 - 104 (UniProt: Q8S307)

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	217649.8
Branched	Number of molecules	4
	Total formula weight	1369.2
Non-Polymers*	Number of molecules	3
	Total formula weight	186.2
All*	Total formula weight	219205.2

*Water molecules are not included.