9M54

Cryo-EM structure of neuropeptide FF receptor 2 complex with NPVF

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Neuropeptide FF receptor 2	polymer	420	48734.1	1	UniProt (Q9Y5X5) Pfam (PF00001)	Homo sapiens (human)	G-protein coupled receptor 74,G-protein coupled receptor HLWAR77,Neuropeptide G-protein coupled receptor
2	B (L)	Neuropeptide VF	polymer	9	969.1	1		synthetic construct
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	354	38975.6	1	UniProt (P54311) Pfam (PF00400)	Rattus norvegicus (Norway rat)	Transducin beta chain 1
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (domestic cattle)	G gamma-I
5	E (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40445.1	1	UniProt (P63097) Pfam (PF00503)	Bos taurus (domestic cattle)	Adenylate cyclase-inhibiting G alpha protein
6	F (S)	scfv16	polymer	247	26277.3	1		Mus musculus

Sequence modifications

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag

A: 1 - 354 (UniProt: P63097)

PDB	External Database	Details
Ala 203	Gly 203	engineered mutation
Ser 326	Ala 326	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	163262.3
All*	Total formula weight	163262.3

*Water molecules are not included.