9M2N

Crystal Structure of the double mutant (Y128A and C222A) of Sortase E mutant from Thermobifida fusca

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Peptidase C60, sortase A and B	polymer	181	20157.3	2	UniProt (Q47MA6)	Thermobifida fusca YX	Peptidase C60, sortase E
2	C, D, H (A, B)	SODIUM ION	non-polymer		23.0	3	Chemie (NA)
3	E, F, G, I, J... (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
4	L, M (A, B)	water	water		18.0	229	Chemie (HOH)

Sequence modifications

A, B: 91 - 262 (UniProt: Q47MA6)

PDB	External Database	Details
Met 90	-	initiating methionine
Ala 128	Tyr 128	engineered mutation
Ala 222	Cys 222	engineered mutation
Leu 263	-	expression tag
Glu 264	-	expression tag
His 265	-	expression tag
His 266	-	expression tag
His 267	-	expression tag
His 268	-	expression tag
His 269	-	expression tag
His 270	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	40314.7
Non-Polymers*	Number of molecules	9
	Total formula weight	441.4
All*	Total formula weight	40756.1

*Water molecules are not included.