9M2F
Structure of neuropeptide FF receptor 1 complex with NPFF
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 366 | 39750.3 | 1 | UniProt (P54311) Pfam (PF00400) | Rattus norvegicus (Norway rat) | Transducin beta chain 1 |
| 2 | B (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (domestic cattle) | G gamma-I |
| 3 | C (L) | neuropeptide FF | polymer | 8 | 934.1 | 1 | synthetic construct | ||
| 4 | D (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40445.1 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 5 | E (R) | Neuropeptide FF receptor 1,Neuropeptide FF receptor 1,Lgbit | polymer | 536 | 59432.6 | 1 | UniProt (Q9GZQ6) Pfam (PF00001) | Homo sapiens (human) | G-protein coupled receptor 147,RFamide-related peptide receptor OT7T022 |
| 6 | F (S) | scfv16 | polymer | 247 | 26277.3 | 1 | synthetic construct |
Sequence modifications
B: 2 - 340 (UniProt: P54311)
A: 1 - 354 (UniProt: P63096)
R: 1 - 363 (UniProt: Q9GZQ6)
R: 380 - 536 (PDB: 9M2F)
| PDB | External Database | Details |
|---|---|---|
| Met -10 | - | initiating methionine |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| Gly 341 | - | expression tag |
| Ser 342 | - | expression tag |
| Ser 343 | - | expression tag |
| Gly 344 | - | expression tag |
| Gly 345 | - | expression tag |
| Gly 346 | - | expression tag |
| Gly 347 | - | expression tag |
| Ser 348 | - | expression tag |
| Gly 349 | - | expression tag |
| Gly 350 | - | expression tag |
| Gly 351 | - | expression tag |
| Gly 352 | - | expression tag |
| Ser 353 | - | expression tag |
| Ser 354 | - | expression tag |
| Gly 355 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 203 | Gly 203 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 364 | - | linker |
| Ser 365 | - | linker |
| Ser 366 | - | linker |
| Gly 367 | - | linker |
| Gly 368 | - | linker |
| Gly 369 | - | linker |
| Gly 370 | - | linker |
| Ser 371 | - | linker |
| Gly 372 | - | linker |
| Gly 373 | - | linker |
| Gly 374 | - | linker |
| Gly 375 | - | linker |
| Ser 376 | - | linker |
| Ser 377 | - | linker |
| Gly 378 | - | linker |
| Val 379 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 174700.5 | |
| All* | Total formula weight | 174700.5 |
