9M1O

Cryo-EM structures of NPFFR2 complex with neuropeptide FF

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Soluble cytochrome b562,Neuropeptide FF receptor 2,Neuropeptide FF receptor 2	polymer	589	67597.0	1	UniProt (Q9Y5X5) Pfam (PF00001)	Escherichia coli	G-protein coupled receptor 74,G-protein coupled receptor HLWAR77,Neuropeptide G-protein coupled receptor
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	366	39750.3	1	UniProt (P54311) Pfam (PF25391)	Rattus norvegicus (Norway rat)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (domestic cattle)	G gamma-I
4	D (L)	Morphine-modulating neuropeptide B	polymer	9	1081.3	1	UniProt (P15507)	synthetic construct
5	E (S)	scfv16	polymer	247	26277.3	1		synthetic construct
6	F (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40445.1	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein

Sequence modifications

R: -54 - 102 (PDB: 9M1O)
R: 103 - 522 (UniProt: Q9Y5X5)

PDB	External Database	Details
His 523	-	expression tag
His 524	-	expression tag
His 525	-	expression tag
His 526	-	expression tag
His 527	-	expression tag
His 528	-	expression tag
His 529	-	expression tag
His 530	-	expression tag
His 531	-	expression tag
His 532	-	expression tag
His 533	-	expression tag
His 534	-	expression tag

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag

L: 1 - 8 (UniProt: P15507)

PDB	External Database	Details
Nh2 9	-	amidation

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Ala 203	Gly 203	engineered mutation
Ser 326	Ala 326	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	183012.1
All*	Total formula weight	183012.1

*Water molecules are not included.