9M0O

Crystal structure of OPTN 138-170 in complex with GTP-bound RAB8A1-176 (Q67L)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (D)	Ras-related protein Rab-8A	polymer	182	21095.3	1	UniProt (P61006) Pfam (PF00071)	Homo sapiens (human)	Oncogene c-mel
2	B (A)	Optineurin	polymer	36	4036.7	1	UniProt (Q96CV9)	Homo sapiens (human)	E3-14.7K-interacting protein,FIP-2,Huntingtin yeast partner L,Huntingtin-interacting protein 7,HIP-7,Huntingtin-interacting protein L,NEMO-related protein,Optic neuropathy-inducing protein,Transcription factor IIIA-interacting protein,TFIIIA-IntP
3	C (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)
4	D (D)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466)
5	E, F, H (D)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL) PubChem (753)
6	G (D)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT) PubChem (175)
7	I, J (D, A)	water	water		18.0	208	Chemie (HOH)

Sequence modifications

D: 1 - 176 (UniProt: P61006)

PDB	External Database	Details
Leu 67	Gln 67	engineered mutation
His 177	-	expression tag
His 178	-	expression tag
His 179	-	expression tag
His 180	-	expression tag
His 181	-	expression tag
His 182	-	expression tag

A: 138 - 170 (UniProt: Q96CV9)

PDB	External Database	Details
Gly 135	-	expression tag
Pro 136	-	expression tag
Leu 137	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	25131.9
Non-Polymers*	Number of molecules	6
	Total formula weight	882.8
All*	Total formula weight	26014.7

*Water molecules are not included.