9M0O

Crystal structure of OPTN 138-170 in complex with GTP-bound RAB8A1-176 (Q67L)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (D)	Ras-related protein Rab-8A	polymer	182	21095.3	1	UniProt (P61006) Pfam (PF00071)	Homo sapiens (human)	Oncogene c-mel
2	B (A)	Optineurin	polymer	36	4036.7	1	UniProt (Q96CV9)	Homo sapiens (human)	E3-14.7K-interacting protein,FIP-2,Huntingtin yeast partner L,Huntingtin-interacting protein 7,HIP-7,Huntingtin-interacting protein L,NEMO-related protein,Optic neuropathy-inducing protein,Transcription factor IIIA-interacting protein,TFIIIA-IntP
3	C (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	D (D)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
5	E, F, H (D)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
6	G (D)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
7	I, J (D, A)	water	water		18.0	208	Chemie (HOH)

Sequence modifications

D: 1 - 176 (UniProt: P61006)

PDB	External Database	Details
Leu 67	Gln 67	engineered mutation
His 177	-	expression tag
His 178	-	expression tag
His 179	-	expression tag
His 180	-	expression tag
His 181	-	expression tag
His 182	-	expression tag

A: 138 - 170 (UniProt: Q96CV9)

PDB	External Database	Details
Gly 135	-	expression tag
Pro 136	-	expression tag
Leu 137	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	25131.9
Non-Polymers*	Number of molecules	6
	Total formula weight	882.8
All*	Total formula weight	26014.7

*Water molecules are not included.