9LW5

Cryo-EM structure of dopamine bound mut-beta2-Adrenergic Receptor beta2R-M16-miniGs-Gbeta1gamma2-Nb35-scFv16 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	384	44650.2	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (S)	scFv 16	polymer	246	26164.1	1		Homo sapiens
5	E (N)	Nanobody 35	polymer	134	14714.3	1		Vicugna pacos
6	F (R)	Beta-2 adrenergic receptor	polymer	357	40660.5	1	UniProt (P07550) Pfam (PF00001)	Homo sapiens (human)	Beta-2 adrenergic receptor beta2R-M16,Beta-2 adrenoreceptor,Beta-2 adrenoceptor
7	G (R)	L-DOPAMINE	non-polymer		153.2	1	Chemie (LDP)

Sequence modifications

A: 26 - 394 (UniProt: P63092)

PDB	External Database	Details
Thr 4	-	expression tag
Leu 5	-	expression tag
Ser 6	-	expression tag
Ala 7	-	expression tag
Glu 8	-	expression tag
Asp 9	-	expression tag
Lys 10	-	expression tag
Ala 11	-	expression tag
Ala 12	-	expression tag
Val 13	-	expression tag
Glu 14	-	expression tag
Arg 15	-	expression tag
Ser 16	-	expression tag
Lys 17	-	expression tag
Met 18	-	expression tag
Asp 49	Gly 49	engineered mutation
Asn 50	Glu 50	engineered mutation
Tyr 63	Leu 63	engineered mutation
Lys 203	Leu 203	engineered mutation
Ala 226	Gly 226	engineered mutation
Asp 249	Ala 249	engineered mutation
Asp 272	Leu 272	engineered mutation
Ser 366	Ala 366	engineered mutation
Ala 372	Ile 372	engineered mutation
Ile 375	Val 375	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 1 - 348 (UniProt: P07550)

PDB	External Database	Details
Asp -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Lys -1	-	expression tag
Gly 0	-	expression tag
Arg 16	Gly 16	variant
Gln 27	Glu 27	variant
Ser 117	Val 117	engineered mutation
Leu 122	Glu 122	engineered mutation
Asn 123	Thr 123	engineered mutation
Val 312	Asn 312	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	173468.3
Non-Polymers*	Number of molecules	1
	Total formula weight	153.2
All*	Total formula weight	173621.5

*Water molecules are not included.