9LRE

Cryo-EM structure of the histamine H4 receptor-Gi protein complex (Overall)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P54311) Pfam (PF25391)	Rattus norvegicus (Norway rat)	Transducin beta chain 1
3	C (E)	scFv16	polymer	260	27720.8	1		Mus musculus
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7547.7	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (domestic cattle)	G gamma-I
5	E (R)	Histamine H4 receptor,Genome polyprotein	polymer	692	77796.9	1	UniProt (Q9H3N8) UniProt (B6F2F5) Pfam (PF00001)	Homo sapiens (human)	H4R,HH4R,AXOR35,G-protein coupled receptor 105,GPRv53,Pfi-013,SP9144
6	F (R)	HISTAMINE	non-polymer		111.1	1	Chemie (HSM)
7	G (R)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

G: 1 - 67 (UniProt: P63212)

PDB	External Database	Details
Ser 68	-	expression tag

R: 2 - 390 (UniProt: Q9H3N8)

PDB	External Database	Details
Met -32	-	initiating methionine
Lys -31	-	expression tag
Thr -30	-	expression tag
Ile -29	-	expression tag
Ile -28	-	expression tag
Ala -27	-	expression tag
Leu -26	-	expression tag
Ser -25	-	expression tag
Tyr -24	-	expression tag
Ile -23	-	expression tag
Phe -22	-	expression tag
Cys -21	-	expression tag
Leu -20	-	expression tag
Val -19	-	expression tag
Phe -18	-	expression tag
Ala -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Lys -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Lys -9	-	expression tag
Gly -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Gly 391	-	linker
Gly 392	-	linker
Ser 393	-	linker
Gly 394	-	linker
Gly 395	-	linker
Gly 396	-	linker
Ser 397	-	linker
Gly 398	-	linker
Gly 399	-	linker
Ser 400	-	linker
Ser 401	-	linker
Gly 402	-	linker
Gly 403	-	linker
Gly 404	-	linker
Ser 405	-	linker
Leu 406	-	linker
Glu 407	-	linker
Val 408	-	linker
Leu 409	-	linker
Phe 410	-	linker
Gln 411	-	linker

R: 412 - 651 (UniProt: B6F2F5)

PDB	External Database	Details
Pro 413	Ser 3	conflict
Lys 619	Ala 209	conflict
His 652	-	expression tag
His 653	-	expression tag
His 654	-	expression tag
His 655	-	expression tag
His 656	-	expression tag
His 657	-	expression tag
His 658	-	expression tag
His 659	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	192223.8
Non-Polymers*	Number of molecules	1
	Total formula weight	111.1
All*	Total formula weight	192334.9

*Water molecules are not included.