9LRB

Cryo-EM structure of the histamine H1 receptor-Gs protein complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	249	28964.7	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P54311) Pfam (PF25391)	Rattus norvegicus (Norway rat)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7547.7	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (domestic cattle)	G gamma-I
4	D (N)	nanobody 35	polymer	137	15015.7	1		unidentified
5	E (R)	Histamine H1 receptor,Genome polyprotein	polymer	802	90333.7	1	UniProt (P35367) UniProt (W8GG88) Pfam (PF00001)	Homo sapiens (human)	H1-R,H1R,HH1R
6	F (R)	HISTAMINE	non-polymer		111.1	1	Chemie (HSM)

Sequence modifications

A: 5 - 195 (UniProt: P63092)

PDB	External Database	Details
Asp 49	Gly 49	conflict
Asn 50	Glu 50	conflict

A: 204 - 384 (UniProt: P63092)

PDB	External Database	Details
Gly 196	-	linker
Gly 197	-	linker
Ser 198	-	linker
Gly 199	-	linker
Gly 200	-	linker
Ser 201	-	linker
Gly 202	-	linker
Gly 203	-	linker
Asp 249	Ala 249	conflict
Asp 252	Ser 252	conflict
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Ala 362	Ile 372	conflict
Ile 365	Val 375	conflict

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

G: 1 - 67 (UniProt: P63212)

PDB	External Database	Details
Ser 68	-	expression tag

R: 1 - 487 (UniProt: P35367)

PDB	External Database	Details
Met -25	-	initiating methionine
Lys -24	-	expression tag
Thr -23	-	expression tag
Ile -22	-	expression tag
Ile -21	-	expression tag
Ala -20	-	expression tag
Leu -19	-	expression tag
Ser -18	-	expression tag
Tyr -17	-	expression tag
Ile -16	-	expression tag
Phe -15	-	expression tag
Cys -14	-	expression tag
Leu -13	-	expression tag
Val -12	-	expression tag
Phe -11	-	expression tag
Ala -10	-	expression tag
Asp -9	-	expression tag
Tyr -8	-	expression tag
Lys -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Lys -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag
Gly 488	-	linker
Gly 489	-	linker
Ser 490	-	linker
Gly 491	-	linker
Gly 492	-	linker
Gly 493	-	linker
Gly 494	-	linker
Ser 495	-	linker
Gly 496	-	linker
Gly 497	-	linker
Ser 498	-	linker
Ser 499	-	linker
Ser 500	-	linker
Gly 501	-	linker
Gly 502	-	linker
Gly 503	-	linker
Ser 504	-	linker
Ser 505	-	linker
Gly 506	-	linker
Gly 507	-	linker
Ser 508	-	linker
Gly 509	-	linker
Gly 510	-	linker
Gly 511	-	linker
Ser 512	-	linker
Gly 513	-	linker
Gly 514	-	linker
Ser 515	-	linker
Gly 516	-	linker
Gly 517	-	linker
Leu 518	-	linker
Glu 519	-	linker
Val 520	-	linker
Leu 521	-	linker
Phe 522	-	linker
Gln 523	-	linker
Gly 524	-	linker
Pro 525	-	linker

R: 526 - 763 (UniProt: W8GG88)

PDB	External Database	Details
Lys 731	Ala 2562	conflict
Ser 764	-	expression tag
Gly 765	-	expression tag
Leu 766	-	expression tag
Arg 767	-	expression tag
Ser 768	-	expression tag
His 769	-	expression tag
His 770	-	expression tag
His 771	-	expression tag
His 772	-	expression tag
His 773	-	expression tag
His 774	-	expression tag
His 775	-	expression tag
His 776	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	180606.2
Non-Polymers*	Number of molecules	1
	Total formula weight	111.1
All*	Total formula weight	180717.4

*Water molecules are not included.