9LR2
Cryo-EM structure of the JN241-9-bound APLNR dimer-Gi complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | B, C (R, Q) | Apelin receptor | polymer | 380 | 42696.3 | 2 | UniProt (P35414) Pfam (PF00001) | Homo sapiens (human) | Angiotensin receptor-like 1,G-protein coupled receptor APJ,G-protein coupled receptor HG11 |
| 3 | D, E (D, C) | JN241-9 | polymer | 130 | 13929.5 | 2 | synthetic construct | ||
| 4 | F (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | Transducin beta chain 1 |
| 5 | G (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 70 | 7729.9 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 6 | H (S) | single Fab chain(scFv16) | polymer | 250 | 26610.6 | 1 | synthetic construct | ||
| 7 | I, Q (R, Q) | 1,2-DIACYL-SN-GLYCERO-3-PHOSHOCHOLINE | non-polymer | 734.0 | 2 | Chemie (PCF) | |||
| 8 | J, K, L, M, N... (R, Q) | CHOLESTEROL | non-polymer | 386.7 | 14 | Chemie (CLR) |
Sequence modifications
A: 1 - 354 (UniProt: P63096)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | engineered mutation |
| Ala 203 | Gly 203 | engineered mutation |
| Ala 245 | Glu 245 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 225921.7 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 6881.2 | |
| All* | Total formula weight | 232802.9 |
