9LQP
Structure of transposase-activated RAG (RAG1 E962N) target capture complex with disordered U-shaped target DNA (TCC-UD)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | V(D)J recombination-activating protein 1 | polymer | 750 | 85765.8 | 2 | UniProt (P15919) | Mus musculus (house mouse) | Transposase-activated RAG1,RAG-1 |
| 2 | B, D (B, D) | Early growth response protein 1,V(D)J recombination-activating protein 2 | polymer | 457 | 51195.1 | 2 | UniProt (P08046) UniProt (P21784) | Mus musculus (house mouse) | EGR-1,Nerve growth factor-induced protein A,NGFI-A,Transcription factor Zif268,Zinc finger protein Krox-24,RAG-2 |
| 3 | E (I) | Target DNA bottom strand | polymer | 59 | 18061.6 | 1 | synthetic construct | ||
| 4 | F (J) | Target DNA top strand | polymer | 59 | 18305.7 | 1 | synthetic construct | ||
| 5 | G (x) | 23RSS bottom strand | polymer | 45 | 13837.9 | 1 | synthetic construct | ||
| 6 | H (M) | 23RSS top strand | polymer | 45 | 13877.9 | 1 | synthetic construct | ||
| 7 | I (y) | 12RSS bottom strand | polymer | 34 | 10456.7 | 1 | synthetic construct | ||
| 8 | J (L) | 12RSS top strand | polymer | 34 | 10461.8 | 1 | synthetic construct | ||
| 9 | K, L (A, C) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) PubChem (32051) |
Sequence modifications
A, C: 261 - 1008 (UniProt: P15919)
B, D: -98 - -11 (UniProt: P08046)
B, D: 1 - 335 (UniProt: P21784)
B, D: 336 - 355 (UniProt: P21784)
| PDB | External Database | Details |
|---|---|---|
| Gly 259 | - | expression tag |
| Pro 260 | - | expression tag |
| Met 848 | Arg 848 | conflict |
| Asn 962 | Glu 962 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly -101 | - | expression tag |
| Pro -100 | - | expression tag |
| Met -99 | - | expression tag |
| Asp -82 | Arg 349 | conflict |
| Ser -80 | Asp 351 | conflict |
| Asn -79 | Glu 352 | conflict |
| Asp -10 | - | linker |
| Gly -9 | - | linker |
| Gly -8 | - | linker |
| Gly -7 | - | linker |
| Asn -6 | - | linker |
| Gly -5 | - | linker |
| Ser -4 | - | linker |
| Gly -3 | - | linker |
| Gly -2 | - | linker |
| Ser -1 | - | linker |
| Gly 0 | - | linker |
B, D: 336 - 355 (UniProt: P21784)
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 358923.3 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 130.8 | |
| All* | Total formula weight | 359054.1 |
