9LQ7
Cryo-EM structure of NTR-bound type III-B CRISPR-Cas effector complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Cmr1 | polymer | 469 | 55340.3 | 1 | UniProt (A0A5M5QTL0) | Bacteroides fragilis | |
| 2 | B (B) | Type III-B CRISPR-associated protein Cas10/Cmr2 | polymer | 600 | 69247.1 | 1 | UniProt (A0A5C6J4N7) | Bacteroides fragilis | |
| 3 | C (C) | CRISPR-associated protein Cmr3 | polymer | 423 | 49059.2 | 1 | UniProt (A0A9X9IKM4) | Bacteroides fragilis | |
| 4 | D, E, F (D, E, F) | Type III-B CRISPR module RAMP protein Cmr4 | polymer | 279 | 31537.0 | 3 | UniProt (A0A9X9NER2) | Bacteroides fragilis | |
| 5 | G, H (G, H) | CRISPR type III-B/RAMP module-associated protein Cmr5 | polymer | 136 | 15273.5 | 2 | UniProt (A0A2M9V7H9) | Bacteroides fragilis | |
| 6 | I (I) | RAMP superfamily protein | polymer | 314 | 35951.9 | 1 | UniProt (A0A2M9V7I0) | Bacteroides fragilis | Type III-B CRISPR module RAMP protein Cmr6 |
| 7 | J (K) | NTR | polymer | 49 | 15672.4 | 1 | Bacteroides fragilis | ||
| 8 | K (J) | crRNA | polymer | 49 | 15671.2 | 1 | Bacteroides fragilis | ||
| 9 | L (B) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
| 10 | M (B) | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 1 | Chemie (SAM) | |||
| 11 | N (B) | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) |
Sequence modifications
C: 45 - 423 (UniProt: A0A9X9IKM4)
D, E, F: 1 - 279 (UniProt: A0A9X9NER2)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Ser 2 | - | expression tag |
| His 3 | - | expression tag |
| His 4 | - | expression tag |
| His 5 | - | expression tag |
| His 6 | - | expression tag |
| His 7 | - | expression tag |
| His 8 | - | expression tag |
| His 9 | - | expression tag |
| His 10 | - | expression tag |
| Asp 11 | - | expression tag |
| Gly 12 | - | expression tag |
| Lys 13 | - | expression tag |
| Pro 14 | - | expression tag |
| Ile 15 | - | expression tag |
| Pro 16 | - | expression tag |
| Asn 17 | - | expression tag |
| Pro 18 | - | expression tag |
| Leu 19 | - | expression tag |
| Leu 20 | - | expression tag |
| Gly 21 | - | expression tag |
| Leu 22 | - | expression tag |
| Asp 23 | - | expression tag |
| Ser 24 | - | expression tag |
| Thr 25 | - | expression tag |
| Gly 26 | - | expression tag |
| Ser 27 | - | expression tag |
| Asp 28 | - | expression tag |
| Gln 29 | - | expression tag |
| Thr 30 | - | expression tag |
| Glu 31 | - | expression tag |
| Asn 32 | - | expression tag |
| Ser 33 | - | expression tag |
| Gly 34 | - | expression tag |
| Glu 35 | - | expression tag |
| Asn 36 | - | expression tag |
| Leu 37 | - | expression tag |
| Tyr 38 | - | expression tag |
| Phe 39 | - | expression tag |
| Gln 40 | - | expression tag |
| Gly 41 | - | expression tag |
| Ala 42 | - | expression tag |
| Asn 43 | - | expression tag |
| Ala 44 | - | expression tag |
| Tyr 279 | Cys 235 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 27 | Asp 27 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 366100.1 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 960.6 | |
| All* | Total formula weight | 367060.7 |
