9LQ6

Cryo-EM structure of adTR-bound type III-B CRISPR-Cas effector complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cmr1	polymer	469	55340.3	1	UniProt (A0A5M5QTL0)	Bacteroides fragilis
2	B (B)	Type III-B CRISPR-associated protein Cas10/Cmr2	polymer	600	69247.1	1	UniProt (A0A5C6J4N7)	Bacteroides fragilis
3	C (C)	CRISPR-associated protein Cmr3	polymer	423	49059.2	1	UniProt (A0A9X9IKM4)	Bacteroides fragilis
4	D, E, F (D, E, F)	Type III-B CRISPR module RAMP protein Cmr4	polymer	279	31537.0	3	UniProt (A0A9X9NER2)	Bacteroides fragilis
5	G, H (G, H)	CRISPR type III-B/RAMP module-associated protein Cmr5	polymer	136	15273.5	2	UniProt (A0A2M9V7H9)	Bacteroides fragilis
6	I (I)	RAMP superfamily protein	polymer	314	35951.9	1	UniProt (A0A2M9V7I0)	Bacteroides fragilis	Type III-B CRISPR module RAMP protein Cmr6
7	J (K)	adTR	polymer	41	13138.9	1		Bacteroides fragilis
8	K (J)	crRNA	polymer	49	15671.2	1		Bacteroides fragilis
9	L (B)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
10	M (B)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
11	N (B)	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)

Sequence modifications

C: 45 - 423 (UniProt: A0A9X9IKM4)

PDB	External Database	Details
Met 1	-	initiating methionine
Ser 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Asp 11	-	expression tag
Gly 12	-	expression tag
Lys 13	-	expression tag
Pro 14	-	expression tag
Ile 15	-	expression tag
Pro 16	-	expression tag
Asn 17	-	expression tag
Pro 18	-	expression tag
Leu 19	-	expression tag
Leu 20	-	expression tag
Gly 21	-	expression tag
Leu 22	-	expression tag
Asp 23	-	expression tag
Ser 24	-	expression tag
Thr 25	-	expression tag
Gly 26	-	expression tag
Ser 27	-	expression tag
Asp 28	-	expression tag
Gln 29	-	expression tag
Thr 30	-	expression tag
Glu 31	-	expression tag
Asn 32	-	expression tag
Ser 33	-	expression tag
Gly 34	-	expression tag
Glu 35	-	expression tag
Asn 36	-	expression tag
Leu 37	-	expression tag
Tyr 38	-	expression tag
Phe 39	-	expression tag
Gln 40	-	expression tag
Gly 41	-	expression tag
Ala 42	-	expression tag
Asn 43	-	expression tag
Ala 44	-	expression tag
Tyr 279	Cys 235	conflict

D, E, F: 1 - 279 (UniProt: A0A9X9NER2)

PDB	External Database	Details
Ala 27	Asp 27	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	363566.7
Non-Polymers*	Number of molecules	3
	Total formula weight	960.6
All*	Total formula weight	364527.2

*Water molecules are not included.