9LN5
Crystal structure of P450revI A241L mutant in complex with reveromycin T
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Putative monooxygenase | polymer | 401 | 44794.1 | 1 | UniProt (G1UDU7) Pfam (PF00067) | Streptomyces sp. SN-593 | Reveromycin T hydroxylase |
| 2 | B (A) | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 1 | Chemie (HEM) | |||
| 3 | C (A) | (2E,4S,5S,6E,8E)-10-{(2R,3S,6S,8R,9S)-9-butyl-8-[(1E,3E)-4-carboxy-3-methylbuta-1,3-dien-1-yl]-3-methyl-1,7-dioxaspiro[5.5]undec-2-yl}-5-hydroxy-4,8-dimethyldeca-2,6,8-trienoic acid | non-polymer | 544.7 | 1 | Chemie (RRM) | |||
| 4 | D, E (A) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 5 | F (A) | water | water | 18.0 | 150 | Chemie (HOH) |
Sequence modifications
A: 6 - 401 (UniProt: G1UDU7)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Ser 2 | - | expression tag |
| His 3 | - | expression tag |
| Met 4 | - | expression tag |
| Leu 5 | - | expression tag |
| Leu 241 | Ala 237 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 44794.1 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1232.1 | |
| All* | Total formula weight | 46026.2 |
