9LMV
Crystal structure of FAST-ACC-T140D/R132G/S160A mutant in complex with mono(2-hydroxyethyl) terephthalic acid
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Poly(ethylene terephthalate) hydrolase | polymer | 263 | 27289.3 | 1 | UniProt (A0A0K8P6T7) | Piscinibacter sakaiensis | PET hydrolase,PETase,PET-digesting enzyme |
| 2 | B (A) | 4-(2-hydroxyethyloxycarbonyl)benzoic acid | non-polymer | 210.2 | 1 | Chemie (C9C) | |||
| 3 | C (A) | water | water | 18.0 | 292 | Chemie (HOH) |
Sequence modifications
A: 30 - 290 (UniProt: A0A0K8P6T7)
| PDB | External Database | Details |
|---|---|---|
| Gly 28 | - | expression tag |
| Ala 29 | - | expression tag |
| Glu 121 | Ser 121 | conflict |
| Gly 132 | Arg 132 | engineered mutation |
| Asp 140 | Thr 140 | engineered mutation |
| Ala 160 | Ser 160 | engineered mutation |
| His 186 | Asp 186 | conflict |
| Ala 212 | Asn 212 | conflict |
| Gln 224 | Arg 224 | conflict |
| Cys 233 | Asn 233 | conflict |
| Ala 280 | Arg 280 | conflict |
| Cys 282 | Ser 282 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 27289.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 210.2 | |
| All* | Total formula weight | 27499.5 |
