9LJW
Structural insights into the polymerase catalyzed FAD-capping of hepatitis C viral RNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (T) | RNA (5'-R(P*CP*GP*UP*CP*U)-3') | polymer | 5 | 1522.9 | 1 | synthetic construct | ||
| 2 | B (A) | RNA-directed RNA polymerase | polymer | 544 | 60626.7 | 1 | UniProt (Q99IB8) | Hepatitis C virus genotype 2a (isolate JFH-1) (HCV) | NS5B,p68 |
| 3 | C (C) | RNA (5'-R(P*GP*AP*C)-3') | polymer | 3 | 934.6 | 1 | synthetic construct | ||
| 4 | D (A) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 5 | E, F (A) | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) | |||
| 6 | G (C) | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 1 | Chemie (FAD) | |||
| 7 | H, I (A, C) | water | water | 18.0 | 6 | Chemie (HOH) |
Sequence modifications
A: 1 - 551 (UniProt: Q99IB8)
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| Gly 15 | Ser 2457 | engineered mutation |
| Gln 86 | Glu 2528 | engineered mutation |
| Gln 87 | Glu 2529 | engineered mutation |
| His 223 | Cys 2665 | engineered mutation |
| Ile 321 | Val 2763 | engineered mutation |
| Gly 444 | Asn 2886 | engineered mutation |
| - | Phe 2887 | deletion |
| - | Glu 2888 | deletion |
| - | Met 2889 | deletion |
| - | Tyr 2890 | deletion |
| - | Gly 2891 | deletion |
| - | Ser 2892 | deletion |
| - | Val 2893 | deletion |
| - | Tyr 2894 | deletion |
| Gly 445 | Ser 2895 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 63084.3 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1338.6 | |
| All* | Total formula weight | 64422.9 |
