9LJ7
Crystal structure of a leaf-branch compost cutinase variant, ICCG-H218S/F222I
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Leaf-branch compost cutinase | polymer | 265 | 28419.0 | 2 | UniProt (G9BY57) | unidentified prokaryotic organism | LC-cutinase,LCC,PET-digesting enzyme,Poly(ethylene terephthalate) hydrolase,PET hydrolase,PETase |
| 2 | C, E, F, G, H (A, B) | ACETATE ION | non-polymer | 59.0 | 5 | Chemie (ACT) | |||
| 3 | D, I (A, B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 4 | J, K (A, B) | water | water | 18.0 | 625 | Chemie (HOH) |
Sequence modifications
A, B: 36 - 293 (UniProt: G9BY57)
| PDB | External Database | Details |
|---|---|---|
| Met 35 | - | initiating methionine |
| Gly 127 | Tyr 127 | conflict |
| Ser 218 | His 218 | engineered mutation |
| Ile 222 | Phe 222 | engineered mutation |
| Cys 238 | Asp 238 | conflict |
| Ile 243 | Phe 243 | conflict |
| Cys 283 | Ser 283 | conflict |
| Leu 294 | - | expression tag |
| Glu 295 | - | expression tag |
| Leu 296 | - | expression tag |
| Val 297 | - | expression tag |
| Pro 298 | - | expression tag |
| Arg 299 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 56837.9 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 375.4 | |
| All* | Total formula weight | 57213.3 |
