9LGM
Cryo-EM structure of GPR4 complexed with Gs in pH8.0
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 248 | 28907.7 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 346 | 38045.6 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (N) | Nanobody 35 | polymer | 157 | 17057.3 | 1 | Lama glama | ||
| 5 | E (R) | G-protein coupled receptor 4 | polymer | 374 | 42688.3 | 1 | UniProt (P46093) Pfam (PF00001) | Homo sapiens (human) | G-protein coupled receptor 6C.l,GPR6C.l |
Sequence modifications
A: 6 - 64 (UniProt: P63092)
A: 204 - 384 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
R: 1 - 354 (UniProt: P46093)
| PDB | External Database | Details |
|---|---|---|
| Asp 49 | Gly 49 | engineered mutation |
| Asn 50 | Glu 50 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 65 | - | linker |
| Gly 66 | - | linker |
| Ser 198 | - | linker |
| Gly 199 | - | linker |
| Gly 200 | - | linker |
| Ser 201 | - | linker |
| Gly 202 | - | linker |
| Gly 203 | - | linker |
| Asp 249 | Ala 249 | conflict |
| Asp 252 | Ser 252 | conflict |
| - | Asn 254 | deletion |
| - | Met 255 | deletion |
| - | Val 256 | deletion |
| - | Ile 257 | deletion |
| - | Arg 258 | deletion |
| - | Glu 259 | deletion |
| - | Asp 260 | deletion |
| - | Asn 261 | deletion |
| - | Gln 262 | deletion |
| - | Thr 263 | deletion |
| Ala 362 | Ile 372 | engineered mutation |
| Ile 365 | Val 375 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ile -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Arg -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Phe 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Glu 355 | - | expression tag |
| Phe 356 | - | expression tag |
| Leu 357 | - | expression tag |
| Glu 358 | - | expression tag |
| Val 359 | - | expression tag |
| Leu 360 | - | expression tag |
| Phe 361 | - | expression tag |
| Gln 362 | - | expression tag |
| Gly 363 | - | expression tag |
| Pro 364 | - | expression tag |
| His 365 | - | expression tag |
| His 366 | - | expression tag |
| His 367 | - | expression tag |
| His 368 | - | expression tag |
| His 369 | - | expression tag |
| His 370 | - | expression tag |
| His 371 | - | expression tag |
| His 372 | - | expression tag |
| His 373 | - | expression tag |
| His 374 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 134560.0 | |
| All* | Total formula weight | 134560.0 |
