9LFN
Cryo-EM structure of the rice isoamylase ISA1-ISA2 heterocomplex incubated with Amylopectin
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Isoamylase 1, chloroplastic | polymer | 777 | 87281.6 | 2 | UniProt (D0TZF0) Pfam (PF02922) Pfam (PF00128) Pfam (PF21156) | Oryza sativa Japonica Group (Japanese rice) | OsISA1,Protein SUGARY-1 |
| 2 | C (C) | Isoamylase 2, chloroplastic | polymer | 800 | 86622.0 | 1 | UniProt (Q6AU80) Pfam (PF02922) Pfam (PF00128) Pfam (PF21156) | Oryza sativa Japonica Group (Japanese rice) | OsISA2 |
| 3 | D, E, F, G (F, D, E, G) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 504.4 | 4 | ||||
| 4 | H, I (H, I) | alpha-L-Glucopyranose-(1-4)-alpha-L-Glucopyranose-(1-4)-alpha-L-Glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 1153.0 | 2 | ||||
| 5 | J, K, L, M, N... (B, C) | alpha-D-glucopyranose | non-polymer | 180.2 | 7 | Chemie (GLC) |
Sequence modifications
A, B: 55 - 803 (UniProt: D0TZF0)
| PDB | External Database | Details |
|---|---|---|
| Met 27 | - | initiating methionine |
| Gly 28 | - | expression tag |
| Ser 29 | - | expression tag |
| Ser 30 | - | expression tag |
| His 31 | - | expression tag |
| His 32 | - | expression tag |
| His 33 | - | expression tag |
| His 34 | - | expression tag |
| His 35 | - | expression tag |
| His 36 | - | expression tag |
| Ser 37 | - | expression tag |
| Ser 38 | - | expression tag |
| Gly 39 | - | expression tag |
| Leu 40 | - | expression tag |
| Val 41 | - | expression tag |
| Pro 42 | - | expression tag |
| Arg 43 | - | expression tag |
| Gly 44 | - | expression tag |
| Ser 45 | - | expression tag |
| His 46 | - | expression tag |
| Ser 47 | - | expression tag |
| Asp 48 | - | expression tag |
| Glu 49 | - | expression tag |
| Val 50 | - | expression tag |
| Asp 51 | - | expression tag |
| Ala 52 | - | expression tag |
| His 53 | - | expression tag |
| Met 54 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 261185.2 | |
| Branched | Number of molecules | 6 |
| Total formula weight | 4323.8 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1261.1 | |
| All* | Total formula weight | 266770.0 |
