9LD5

Crystal structure of LSSmOrange under cryogenic temperature at pH 8.0

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	mOrange	polymer	241	27805.4	1	UniProt (D0VWW2)	Discosoma sp.
2	B (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
3	C (A)	water	water		18.0	176	Chemie (HOH)

Sequence modifications

A: -4 - 231 (UniProt: D0VWW2)

PDB	External Database	Details
Val 44	Ala 49	engineered mutation
Ofm 66	Phe 70	chromophore
Ofm 66	Gly 71	chromophore
Ofm 66	Tyr 72	chromophore
Leu 83	Phe 88	engineered mutation
Met 143	Trp 148	engineered mutation
Asp 161	Ile 166	engineered mutation
Leu 163	Met 168	engineered mutation
Asp 196	Gly 201	engineered mutation
Leu 232	-	expression tag
Glu 233	-	expression tag
His 234	-	expression tag
His 235	-	expression tag
His 236	-	expression tag
His 237	-	expression tag
His 238	-	expression tag
His 239	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27805.4
Non-Polymers*	Number of molecules	1
	Total formula weight	62.1
All*	Total formula weight	27867.5

*Water molecules are not included.