9L8L
Structure of GPCR megacomplex bound to agonists
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45769.5 | 1 | UniProt (P04896) Pfam (PF00503) | Bos taurus (domestic cattle) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 351 | 38744.4 | 1 | UniProt (P54311) Pfam (PF25391) | Rattus norvegicus (Norway rat) | Transducin beta chain 1 |
| 3 | C (C) | Beta-arrestin-1 | polymer | 392 | 44140.3 | 1 | UniProt (P49407) Pfam (PF00339) Pfam (PF02752) | Homo sapiens (human) | Arrestin beta-1,Non-visual arrestin-2 |
| 4 | D (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 68 | 7563.8 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (domestic cattle) | G gamma-I |
| 5 | E (R) | Beta-2 adrenergic receptor | polymer | 421 | 47584.5 | 1 | UniProt (P07550) Pfam (PF00001) | Homo sapiens (human) | Beta-2 adrenoreceptor,Beta-2 adrenoceptor |
| 6 | F (C) | (3S,8S,9S,12S)-3,12-BIS(1,1-DIMETHYLETHYL)-8-HYDROXY-4,11-DIOXO-9-(PHENYLMETHYL)-6-[[4-(2-PYRIDINYL)PHENYL]METHYL]-2,5, 6,10,13-PENTAAZATETRADECANEDIOIC ACID DIMETHYL ESTER | non-polymer | 704.9 | 1 | Chemie (DR7) PubChem (148192) | |||
| 7 | G (R) | 8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one | non-polymer | 370.4 | 1 | Chemie (P0G) PubChem (49837874) |
Sequence modifications
B: 2 - 340 (UniProt: P54311)
R: 1 - 413 (UniProt: P07550)
| PDB | External Database | Details |
|---|---|---|
| Met -10 | - | initiating methionine |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asp -7 | - | expression tag |
| Tyr -6 | - | expression tag |
| Lys -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Arg 16 | Gly 16 | variant |
| Gln 27 | Glu 27 | variant |
| Arg 273 | Lys 273 | engineered mutation |
| Ser 276 | Gly 276 | engineered mutation |
| Val 277 | Ile 277 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 183802.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 1075.3 | |
| All* | Total formula weight | 184877.8 |
