9L8L
Structure of GPCR megacomplex bound to agonists
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45769.5 | 1 | UniProt (P04896) Pfam (PF00503) | Bos taurus (domestic cattle) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 351 | 38744.4 | 1 | UniProt (P54311) Pfam (PF25391) | Rattus norvegicus (Norway rat) | Transducin beta chain 1 |
| 3 | C (C) | Beta-arrestin-1 | polymer | 392 | 44140.3 | 1 | UniProt (P49407) Pfam (PF00339) Pfam (PF02752) | Homo sapiens (human) | Arrestin beta-1,Non-visual arrestin-2 |
| 4 | D (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 68 | 7563.8 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (domestic cattle) | G gamma-I |
| 5 | E (R) | Beta-2 adrenergic receptor | polymer | 421 | 47584.5 | 1 | UniProt (P07550) Pfam (PF00001) | Homo sapiens (human) | Beta-2 adrenoreceptor,Beta-2 adrenoceptor |
| 6 | F (C) | (3S,8S,9S,12S)-3,12-BIS(1,1-DIMETHYLETHYL)-8-HYDROXY-4,11-DIOXO-9-(PHENYLMETHYL)-6-[[4-(2-PYRIDINYL)PHENYL]METHYL]-2,5, 6,10,13-PENTAAZATETRADECANEDIOIC ACID DIMETHYL ESTER | non-polymer | 704.9 | 1 | Chemie (DR7) PubChem (21696352) PubChem (22940470) PubChem (148192) PubChem (44182297) PubChem (22940469) PubChem (53667683) PubChem (56971648) PubChem (66587081) PubChem (59651325) PubChem (60098916) PubChem (71313511) PubChem (71306984) PubChem (90944682) PubChem (93971972) PubChem (92402749) PubChem (93971971) PubChem (93971973) PubChem (99650408) PubChem (6101863) PubChem (6540550) PubChem (6540690) PubChem (9962872) PubChem (131839569) PubChem (134694218) PubChem (124636677) PubChem (138393803) PubChem (138393804) PubChem (144108234) PubChem (144517104) PubChem (173493799) | |||
| 7 | G (R) | 8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one | non-polymer | 370.4 | 1 | Chemie (P0G) PubChem (45483813) PubChem (49837874) |
Sequence modifications
B: 2 - 340 (UniProt: P54311)
R: 1 - 413 (UniProt: P07550)
| PDB | External Database | Details |
|---|---|---|
| Met -10 | - | initiating methionine |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asp -7 | - | expression tag |
| Tyr -6 | - | expression tag |
| Lys -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Arg 16 | Gly 16 | variant |
| Gln 27 | Glu 27 | variant |
| Arg 273 | Lys 273 | engineered mutation |
| Ser 276 | Gly 276 | engineered mutation |
| Val 277 | Ile 277 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 183802.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 1075.3 | |
| All* | Total formula weight | 184877.8 |
