9L84

Structure of beta1-AR-Gs complex bound to epinephrine and an allosteric modulator

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45769.5	1	UniProt (P04896)	Bos taurus (domestic cattle)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P54311)	Rattus norvegicus (Norway rat)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7563.8	1	UniProt (P63212)	Bos taurus (domestic cattle)	G gamma-I
4	D (N)	NB35	polymer	138	15140.7	1		Lama glama
5	E (R)	Beta-1 adrenergic receptor	polymer	477	51275.5	1	UniProt (P08588)	Homo sapiens (human)	Beta-1 adrenoreceptor,Beta-1 adrenoceptor
6	F (R)	(3S,8S,9S,12S)-3,12-BIS(1,1-DIMETHYLETHYL)-8-HYDROXY-4,11-DIOXO-9-(PHENYLMETHYL)-6-[[4-(2-PYRIDINYL)PHENYL]METHYL]-2,5, 6,10,13-PENTAAZATETRADECANEDIOIC ACID DIMETHYL ESTER	non-polymer		704.9	1	Chemie (DR7)
7	G (R)	L-EPINEPHRINE	non-polymer		183.2	1	Chemie (ALE)

Sequence modifications

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	158493.9
Non-Polymers*	Number of molecules	2
	Total formula weight	888.1
All*	Total formula weight	159382.0

*Water molecules are not included.