9L80
Structure of GPR119-Gs complex bound to an allosteric modulator
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 245 | 28299.1 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 351 | 38744.4 | 1 | UniProt (P54311) Pfam (PF25391) | Rattus norvegicus (Norway rat) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 68 | 7563.8 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (domestic cattle) | G gamma-I |
| 4 | D (N) | NB35 | polymer | 138 | 15140.7 | 1 | Lama glama | ||
| 5 | E (R) | Glucose-dependent insulinotropic receptor | polymer | 359 | 39675.8 | 1 | UniProt (Q8TDV5) Pfam (PF00001) | Homo sapiens (human) | G-protein coupled receptor 119 |
| 6 | F (S) | scFv16 | polymer | 259 | 27784.9 | 1 | Mus musculus | ||
| 7 | G (R) | (3S,8S,9S,12S)-3,12-BIS(1,1-DIMETHYLETHYL)-8-HYDROXY-4,11-DIOXO-9-(PHENYLMETHYL)-6-[[4-(2-PYRIDINYL)PHENYL]METHYL]-2,5, 6,10,13-PENTAAZATETRADECANEDIOIC ACID DIMETHYL ESTER | non-polymer | 704.9 | 1 | Chemie (DR7) |
Sequence modifications
A: 204 - 384 (UniProt: P63092)
B: 2 - 340 (UniProt: P54311)
R: 1 - 335 (UniProt: Q8TDV5)
| PDB | External Database | Details |
|---|---|---|
| Gly 9 | - | expression tag |
| Cys 10 | - | expression tag |
| Thr 11 | - | expression tag |
| Leu 12 | - | expression tag |
| Ser 13 | - | expression tag |
| Ala 14 | - | expression tag |
| Glu 15 | - | expression tag |
| Asp 16 | - | expression tag |
| Lys 17 | - | expression tag |
| Ala 18 | - | expression tag |
| Ala 19 | - | expression tag |
| Val 20 | - | expression tag |
| Glu 21 | - | expression tag |
| Arg 22 | - | expression tag |
| Ser 23 | - | expression tag |
| Lys 24 | - | expression tag |
| Met 25 | - | expression tag |
| Ile 26 | - | expression tag |
| Glu 27 | - | expression tag |
| Lys 28 | - | expression tag |
| Gln 29 | - | expression tag |
| Leu 30 | - | expression tag |
| Gln 31 | - | expression tag |
| Lys 32 | - | expression tag |
| Asp 33 | - | expression tag |
| Lys 34 | - | expression tag |
| Gln 35 | - | expression tag |
| Val 36 | - | expression tag |
| Tyr 37 | - | expression tag |
| Arg 38 | - | expression tag |
| Ala 39 | - | expression tag |
| Thr 40 | - | expression tag |
| His 41 | - | expression tag |
| Arg 42 | - | expression tag |
| Leu 43 | - | expression tag |
| Leu 44 | - | expression tag |
| Leu 45 | - | expression tag |
| Leu 46 | - | expression tag |
| Gly 47 | - | expression tag |
| Ala 48 | - | expression tag |
| Asp 49 | - | expression tag |
| Asn 50 | - | expression tag |
| Ser 51 | - | expression tag |
| Gly 52 | - | expression tag |
| Lys 53 | - | expression tag |
| Ser 54 | - | expression tag |
| Thr 55 | - | expression tag |
| Ile 56 | - | expression tag |
| Val 57 | - | expression tag |
| Lys 189 | - | expression tag |
| Gln 190 | - | expression tag |
| Met 191 | - | expression tag |
| Arg 192 | - | expression tag |
| Ile 193 | - | expression tag |
| Leu 194 | - | expression tag |
| His 195 | - | expression tag |
| Gly 196 | - | expression tag |
| Gly 197 | - | expression tag |
| Ser 198 | - | expression tag |
| Gly 199 | - | expression tag |
| Gly 200 | - | expression tag |
| Ser 201 | - | expression tag |
| Gly 202 | - | expression tag |
| Gly 203 | - | expression tag |
| Asp 249 | Ala 249 | engineered mutation |
| Asp 252 | Ser 252 | engineered mutation |
| - | Met 255 | deletion |
| - | Val 256 | deletion |
| - | Ile 257 | deletion |
| - | Arg 258 | deletion |
| - | Glu 259 | deletion |
| - | Asp 260 | deletion |
| - | Asn 261 | deletion |
| - | Gln 262 | deletion |
| - | Thr 263 | deletion |
| - | Asn 264 | deletion |
| Asp 262 | Leu 272 | engineered mutation |
| Ala 362 | Ile 372 | engineered mutation |
| Ile 365 | Val 375 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -10 | - | initiating methionine |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -23 | - | initiating methionine |
| Lys -22 | - | expression tag |
| Thr -21 | - | expression tag |
| Ile -20 | - | expression tag |
| Ile -19 | - | expression tag |
| Ala -18 | - | expression tag |
| Leu -17 | - | expression tag |
| Ser -16 | - | expression tag |
| Tyr -15 | - | expression tag |
| Ile -14 | - | expression tag |
| Phe -13 | - | expression tag |
| Cys -12 | - | expression tag |
| Leu -11 | - | expression tag |
| Val -10 | - | expression tag |
| Phe -9 | - | expression tag |
| Ala -8 | - | expression tag |
| Asp -7 | - | expression tag |
| Tyr -6 | - | expression tag |
| Lys -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 157208.7 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 704.9 | |
| All* | Total formula weight | 157913.5 |
